商品介绍
| Description | |
|---|---|
| CatalogueNumber | HTS002M |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | ChemiScreen™CXCR2MembranePreparation |
| Overview | TRANSFECTION:Full-lengthhumanCXCR2CDNA(AccessionNumber:M73969) |
| BackgroundInformation | CXCR2isa7-TMG-proteincoupledreceptorthatbindstothechemokinesGROalpha,GRObeta,GROgamma,IL-8,ENA-78,NAP-2andGCP-2(OlsonandLey,2002).Neutrophils,mastcellsandmicrovascularendothelialcellsexpressCXCR2,andinteractionsofCXCR2withitsligandspromoteschemotaxisofthesecelltypes(Heidemannetal.,2003;Nilssonetal.,1999;Whiteetal.,1998).StudieswithmicelackingCXCR2indicatethatCXCR2promotesgrowthofprimarytumorsandsecondarymetastases(Keaneetal.,2004),andplaysanessentialroleinhyperoxia-inducedlunginjury(Sueetal.,2004).Inaddition,cytomegalovirusencodesaCXCR2-bindingchemokine,vCXC-1,thatpromotesneutrophilmigrationtoinfectedcells(Penfoldetal.,1999).Chemicon"sCXCR2MembranePrepsareidealtoolsforscreeningforantagoNISTsofinteractionsbetweenCXCR2anditsligands.RADIolabeledIL-8bindstotheCXCR2membraneswithaKdof0.075nM.With0.1nM125I-labeledIL-8,a5-foldwindowofspecificbindingisobtained. |
| ProductInformation | |
|---|---|
| Format | Membranes |
| Presentation | Membraneswereadjustedto0.5mgprotein/mlin50mMTrispH7.4,10%glyceroland1%BSA,rapidlyfrozen,andstoredat-80°C.Onevialtypicallycontains1000units,whereaunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificbindingof125I-labeledIL-8(0.1nMinputconcentration). |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfrozenat-70°Cforupto2years.Donotfreezeandthaw. |
| Applications | |
|---|---|
| Application | HumanCXCR2/IL-8bGPCRmembranepreparationforRadioligandbindingAssays. |
| KeyApplications |
|
| ApplicationNotes | Radioligandbindingassay. |
| BIOLOGicalInformation | |
|---|---|
| Concentration | 0.5mg/mL |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Thisreceptoralsobindstochemokine(C-X-Cmotif)ligand1(CXCL1/MGSA),aproteinwithmelanomagrowthstimulatingactivity,andhasbeenshowntobeamajorcomponentrequiredforserum-dependentmelanomacellgrowth.Thisreceptormediatesneutrophilmigrationtositesofinflammation.TheangiogeniceffectsofIL8inintestinalmicrovascularendothelialcellsarefoundtobemediatedbythisreceptor.Knockoutstudiesinmicesuggestedthatthisreceptorcontrolsthepositioningofoligodendrocyteprecursorsindevelopingspinalcordbyarrestingtheirmigration.Thisgene,IL8RA,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36. |
| GeneSymbol |
|
| GPCRClass | A |
| HostCells | Chem-1 |
| ProteinTarget | CXCR2/IL-8b |
| TargetSub-Family | Chemokine |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P25025#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoGRO/MGSAandNAP-2alsowithahighaffinity. SIZE:360aminoacids;40759Da SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein. PTM:Phosphorylateduponligandbinding;whichisrequiredfordesensitization. SIMILARITY:SwissProt:P25025##BelongstotheG-proteincoupledreceptor1family. |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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