商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3418 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-MAP2Antibody,cloneAP20 |
| AlternateNames |
|
| BackgroundInformation | MAP-2(microtubuleassociatedprotein-2)isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainneuronmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles.MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2.MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds.(Caceres,1986;Dotti,1987). |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG1inbuffercontaining0.02MPhosphatebuffer,pH7.6,0.25MNaClwith0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor6monthsat2-8°Cinundilutedaliquotsfromdateofreceipt. Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200µLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer’scap. |
| Applications | |
|---|---|
| Application | Anti-MAP2Antibody,cloneAP20detectslevelofMAP2&hasbeenpublished&validatedforuseinIH&WB. |
| KeyApplications |
|
| ApplicationNotes | WesternBlot: 60ng/mL.InSDS-PAGE,MAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately280-300kDrepresentingtheMAP-2Aand-2Bisoformsbutnotthe70kDaMAP-2Cisoform.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier).Usersshouldrun4%-20%SDS-PAGEgrADIentgels. Immunohistochemistry: 5µg/mL(4%PFAfixedfrozensections). Immunohistochemistry: 5µg/mLofapreviouslotwasused.Anti-MAP-2canbeusedtostaintissue(brainorspinalcord)fixedwithparaformaldehyde. Optimalworkingconcentrationsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bovinebrainmicrotubuleprotein. |
| Clone | AP20 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | MAB3418bindsspecificallytoMAP-2. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Ratandmouse.Expectedtocross-reactwithhuman,cow,chicken,quail,andXenopus. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstABIlizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed. |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules. SIZE:1827aminoacids;199539Da PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats. |
| MolecularWeight | 230kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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