Description | |
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CatalogueNumber | FCCS025132 |
TradeName |
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Description | FlowCellect™p38StressPathwayActivationDetectionKit |
Overview | Millipore’sFlowCellect™p38StressPathwayActivationDetectionKitisdesignedtoenablearesearcheraquickandeasywaytodetectthephosphorylationstateofP38αandATF2byflowcytometry.Millipore’sFlowCellect™p38StressPathwayActivationKitwasdevelopedandtestedusingtheactivatingreagent,anisomycin,inJurkatcellsbutthekitcanbeusedwithotherhumancelllinestodeterminetheeffectofstressstimuliandchemicalreagentsthatcaninduceP38pathwaysignaling. Millipore’sFlowCellect™p38StressPathwayActivationDetectionKitcontainssufficientreagentsfor252-colortests.Thekitincludestwooptimizedfluorescentlabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.Detailedassayinstructionsareincludedtoassistinyouranalysisandtoensurethatthecorrectcellconcentrationisobtainedduringacquisitionofsampledata. |
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BackgroundInformation | p38isamemberofthemitogenactivatedkinasefamilythatrespondstostressstimuli,suchasinflammatorycytokines,ultravioletirrADIation,heatshock,andosmoticshock.p38isinvolvedincelldifferentiationandapoptosis.Morerecently,P38hasbeenshowntohavearoleincellcyclecheckpointsandDNAdamagepathways.Theupstreamkinases,MKK3,MKK6andSEKactivatep38αbyphosphorylationatThr180andTyr182. Onceactivated,p38αMAPkinasehasbeenshowntophosphorylateandactivateanumberofproteinsincludingMAPKAPkinase2andthetranscriptionfactorsATF-2,MEF2andMSK1. |
ProductInformation | |
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Components |
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Detectionmethod | Fluorescent |
StorageandShippingInformation | |
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StorageConditions | 4-8°Cforantibodiesandbuffers |
BIOLOGicalInformation | |
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Host | Mouse |
SpeciesReactivity |
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AntibodyType | MonoclonalAntibody |
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MaterialsInformation |
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MaterialsInformation |
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