Description | |
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CatalogueNumber | 01-185 |
BrandFamily | Upstate |
TradeName |
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Description | VEGFProtein,Humanrecombinant |
ProductInformation | |
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Formulation | |
Presentation | 10µgofVEGFlyophilizedproteinfrombuffercontaining25mMHEPESand150mMNaCl,pH7.0.Carrierfree. |
StorageandShippingInformation | |
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StorageConditions | Thelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewateror25mMHEPESand150mMNaCl,pH7.0.Reconstitutedmaterialcanbestoredat2°-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles. Thisproductisacidstable.Diluteineither0.1%TFAor4mMHCl,containing25ugBSAperugVEGFtomaintainstABIlity.Reducingreagents(suchasdithiothrietolor2-mercaptoethanol)inhibitVEGFactivity. |
Applications | |
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Application | ThehumanrecombinantVEGFProteinisavailableina10µgformat. |
KeyApplications |
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ApplicationNotes | VEGFProliferationAssay 1.Priortouseinthisassay,maintainHUVEcellsinF-12Kmediumsupplementedwith10%(v/v)FBS,1%penicillin-streptomycin-glutamine,0.1mg/mlheparinand0.05mg/mlendothelialcellgrowthsupplement(ECGS). 2.ForassayofVEGF,subcultureHUVEcellsintoa96-wellplateatadensityof4,000cellsperwellinthegrowthmediumovernight. 3.AddVEGFtoafinalconcentrationrangeof0.2,0.4,0.8,1.6,3.2,6.2,12.5,25and50ng/ml.AddVEGFdiluenttoonesetofwellsasthenegativecontrol. 4.Fourdayslater,add20ulofPromegaSubstrateCellTiter96AqueousOneSolutionReagenttoeachwell. 5.Incubateat37Cfor2hrsandreadatOD490nm. |
BIOLOGicalInformation | |
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Source | RecombinanthumanDNAexpressedinbaculovirusinfectedSf9cells |
EntrezGeneNumber | |
EntrezGeneSummary | ThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms. |
GeneSymbol |
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PurificationMethod | Sterilizedthrougha0.2micronmembranefilter |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145. SIZE:232aminoacids;27042Da SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity). SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin. TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon. SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily. |
StemCellType |
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PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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