Description | |
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CatalogueNumber | 04-218 |
BrandFamily | Upstate |
TradeName |
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Description | Anti-CREBAntibody,cloneE306,rabbitmonoclonal |
AlternateNames |
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BackgroundInformation | CREB,cyclicAMP(cAMP)responseelement-bindingprotein,isaubiquitouslyexpressedbasicleucinezippertranscriptionfactorthatfunctionsinglucosehomeostasisandstimulatestheexpressionofnumerousgenesinresponsetogrowthfactors,hormones,neurotransmitters,ionfluxes,stresssignals,andhasbeenimplicatedinmemorybecauseifitsapparentroleinsynapticplasticity.CREBfunctionseitherbyitselforwithrelatedfamilymembers,whichincludeCREB,CREM,andATF-1,byforminghomo-andheterodimersthroughtheirbasicleucinezipper(bZIP)domains.TheCREBsignalingpathwayisactivatedbyextracellularligandsthatbindtocellsurfacereceptorsandrelaytheirmessagethroughvariousintracellularsecondmessengersresultingultimatelyintheactivationofnuclearCREBthroughphosphorylationofthetransactivationdomainatserine1332,4.TheclassicalmodelofCREBactivationisthroughPKA(cAMP-dependentproteinkinase).Inthenon-activestate,PKAresidesinthecytoplasmasaninactiveheterotetramerofpairedregulatoryandcatalyticsubunits.StimulationofcAMPcausesthereleasesofthecatalyticsubunitsofPKAthatpassivelydiffuseintothenucleusandinitiatesgeneexpressionbyphosphorylatingCREBatitstransactivationdomainonserine1331,2,3,4.CREBmediatestheactivationofcAMP-responsivegenesbybindingasadimertoaconservedcAMP-responsiveelement(CRE),5’-TGACGTCA-3’,locatedwithinthepromoterregionoftargetgenes.Targetgeneactivationisterminatedbytheserine/threoninephosphatasesPP-1-mediateddephospho-rylationofCREB. |
ProductInformation | |
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Format | CultureSupernatant |
Control |
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Presentation | 100μLofrabbitmonoclonalIgGin60%storagebuffer(50mMTris-Glycine(pH7.4),0.15MNaCl,0.01%sodiumazideand0.05%BSA)and40%glycerol. |
StorageandShippingInformation | |
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StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
BIOLOGicalInformation | |
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Immunogen | KLHconjugatedtoCREBlinearpeptide. |
Epitope | Internal |
Clone | E306 |
Host | Rabbit |
Specificity | ThisantibodyrecognizesCREB. |
Isotype | IgG |
SpeciesReactivity |
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SpeciesReactivityNote | Proventoreactwithhuman,ratandmouse. |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
GeneSymbol |
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UniProtNumber | |
UniProtSummary | Function:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity. Subunitstructure:InteractswithPPRC1.BindsDNAasadimer.Thisdimerisstabilizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity. Subcellularlocation:Nucleus. Post-translationalmodification:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR. Sequencesimilarities:BelongstothebZIPfamily. Contains1bZIPdomain. Contains1KID(kinase-inducIBLe)domain. |
MolecularWeight | ~43kDa |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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Millipore常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |