商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-600 |
| BrandFamily | Upstate |
| TradeName |
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| Description | ChIPAb+CREB-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal |
| Overview | EverylotoftheChIPAb+lineofantibodiesisindividuallyvalidatedforchromatinprecipitation,inordertoguaranteesuccessfulChIPassayseverytime.Eachantibodyincludesacontrolprimersetforperformanceconfirmation.CREBantibodyandthenegativecontrolantibody(rabbitnormalIgG)canbeusedtodemonstratethattheCREBantibodyisfunctionallyvalidatedintheprecipitationofCREBassociatedchromatin. TheqPCRprimersincludedflanktheCREBbindingsiteinhumanNR4A2promoter. |
| AlternateNames |
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| BackgroundInformation | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+CREB,antibody/primersetincludestheCREB,antibody,anegativecontrolantibody(purifiedrabbitIgG),andqPCRprimerswhichamplifya64bpregionflankingtheCREofthehumancFosgene.TheCREBandnegativecontrolculturedsupernatantaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofCREBassociatedchromatin. |
| ProductInformation | |
|---|---|
| Components |
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| Format | CultureSupernatant |
| Control |
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| Presentation | 25assaysperset.Recommendeduse:~10μLantibodyperchromatinimmunoprecipitation(dependentuponbiologicalcontext). |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| BiologicalInformation | |
|---|---|
| Immunogen | CREBpurifiedantibodyismadeagainstaKLH-conjugatedsyntheticpeptidecorre-spondingtoaminoacids5-24ofhumanCREB. |
| Epitope | a.a.5-24 |
| Clone | NL904 |
| Host | Rabbit |
| Specificity | RecognizesCREB,MW~43kDa. |
| Isotype | IgG |
| SpeciesReactivity |
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| SpeciesReactivityNote | Human,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity. SUBUNITSTRUCTURE:InteractswithPPRC1.BindsDNAasadimer.ThisdimerisstABIlizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity. SUBCELLULARLOCATION:Nucleus. PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR. INVOLVEMENTINDISEASE:AchromosomalaberrationinvolvingCREB1isassociatedwithangiomatoidfibroushistiocytoma(AFH)[MIM:612160].Translocationt(2;22)(q33;q12)withCREB1generatesaEWSR1/CREB1fusiongenethatismostcommongeneticabnormalityinthistumortype. SEQUENCESIMILARITIES:BelongstothebZIPfamily.Contains1bZIPdomain.Contains1KID(kinase-inducIBLe)domain. |
| MolecularWeight | ~43kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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