产品名称：Millipore/MAB1592 | Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1/MAB1592/100 µL
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB1592 |
| Replaces | 04-1061 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-NeurofilamentH&MAntibody,phosphorylated,cloneNP1 |
| BackgroundInformation | NeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection. |
| ProductInformation | |
|---|---|
| Format | Ascites |
| Presentation | Liquid.Containsnopreservative. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforuptosixmonths.Avoidrepeatedfreeze/thawcycles. |
| Applications | |
|---|---|
| Application | DetectNeurofilamentH&MusingthisAnti-NeurofilamentH&MAntibody,phosphorylated,cloneNP1validatedforuseinELISA,FC,RIA,WB,IC,IH(P). |
| KeyApplications |
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| ApplicationNotes | Westernblot:recognizes,200kDa,and150kDaNF-HandNF-Mbandsprimarily.Stainingisremovedwithphosphatasetreatment.BecauseNF-Lalsocontainslysine-serinePO4sites,NP1maycrossreactwithNF-Laswell,butthishasnotbesubstantiatedintraditionalwesterns. Immunohistochemistry.Worksonparaffinembedded,formalinfixedandfrozenacteonefixedtissuesections.1:50-1:500forimmunofluorescence. ImmunocytochemistryonPC12,TERA-2andotherneuronallines. FACSELISA/RIA Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bovineneurofilaments |
| Epitope | phosphorylated |
| Clone | NP1 |
| Host | Mouse |
| Specificity | RecognizestheKSPrepeatsofthephosphorylatedformsofneurofilament-M(NF-M)andneurofilament-H(NF-H),becauseNF-LdoescontainafewoftheseLysine-serine-PO4-prolinerepeatssomecrossreactivitytoNF-Lmaybeseenatveryhighantibodyconcentrations. |
| Isotype | IgG1 |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12036#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.NF-HhasanimportantfunctioninmatureaxonsthatisnotsubservedbythetwosmallerNFproteins. SIZE:1026aminoacids;112480Da PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFHisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFHresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction. DISEASE:SwissProt:P12036#DefectsinNEFHareacauseofsusceptibilitytoamyotrophiclateralsclerosis(ALS)[MIM:105400].ALSisaneurodegenerativedisorderaffectingupperandlowermotorneurons,andresultinginfatalparalysis.Sensoryabnormalitiesareabsent.Deathusuallyoccurswithin2to5years.Theetiologyislikelytobemultifactorial,involvingbothgeneticandenvironmentalfactors. SIMILARITY:SwissProt:P12036##Belongstotheintermediatefilamentfamily. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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