Millipore/17-470 | phospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit/17-470/96 assays,Millipore,,Millipore,密理博,merckmillipore,Millipore公司代理生物计划 millipore超滤膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/17-470 | phospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit/17-470/96 assays

产品编号：17-470

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	17-470
BrandFamily	Upstate
TradeName	STAR Upstate
Description	phospho-Met(Tyr1230/Tyr1234/Tyr1235)STARELISAKit
BackgroundInformation	TestPrinciple: TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheMetplateiscoatedwithaspecificmousemonoclonalanti-Metcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMetantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-Met(Tyr1230/Tyr1234/Tyr1235)antibodytodetectthecapturedphospho-Met(Tyr1230/Tyr1234/Tyr1235)ontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve. MetBackground: MetisatyrosinekinasereceptorforHepatocyteGrowthFactor(HGF),thoughttostimulatemultiplecellularprocessesincludingcellproliferation,differentiation,cellmigrationandtumOrigenesis.ChronicstimulationofMetoncancercellsisthoughttoplayaroleinmetastasis.
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	1.MetCapturePlate:(PartNo.17-470A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. 2.Anti-phospho-Met(Tyr1230/Tyr1234/Tyr1235)detectionantibody:(PartNo.17-470B)Onebottle(11mL)ofanti-phospho-Met(Tyr1230/Tyr1234/Tyr1235)detectionantibodycontainingsodiumazide,readytouse. 3.ELISADiluent:(PartNo.17-470C)Onebottle(25mL)ofELISADiluentsodiumazide,readytouse. 4.25XELISAWashBuffer:(PartNo.17-470D)Onebottle(50mL)of25XELISAWashBuffer. 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-470E)Onevial(125µL)of100Xanti-rabbitHRPconjugatecontainingthimerosol. 6.HRPDiluent:(PartNo.17-470F)Onebottle(25mL)ofHRPdiluentcontainingthimerosol. 7.TMBSolution:(PartNo.17-470G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse. 8.StopSolution:(PartNo.17-470H)Onebottle(25mL)ofstopsolution,readytouse. 9.phospho-Met(Tyr1230/Tyr1234/Tyr1235)Standard:(PartNo.17-470I)Twovialsofphospho-Met(Tyr1230/Tyr1234/Tyr1235)standard,lyophilized. 10.PlateCovers:Twoplatecovers.
Detectionmethod	ChromogenicColorimetric

ProductInformation

Components

1.MetCapturePlate:(PartNo.17-470A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
2.Anti-phospho-Met(Tyr1230/Tyr1234/Tyr1235)detectionantibody:(PartNo.17-470B)Onebottle(11mL)ofanti-phospho-Met(Tyr1230/Tyr1234/Tyr1235)detectionantibodycontainingsodiumazide,readytouse.
3.ELISADiluent:(PartNo.17-470C)Onebottle(25mL)ofELISADiluentsodiumazide,readytouse.
4.25XELISAWashBuffer:(PartNo.17-470D)Onebottle(50mL)of25XELISAWashBuffer.
5.Anti-RabbitIgGHRPconjugate:(PartNo.17-470E)Onevial(125µL)of100Xanti-rabbitHRPconjugatecontainingthimerosol.
6.HRPDiluent:(PartNo.17-470F)Onebottle(25mL)ofHRPdiluentcontainingthimerosol.
7.TMBSolution:(PartNo.17-470G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
8.StopSolution:(PartNo.17-470H)Onebottle(25mL)ofstopsolution,readytouse.
9.phospho-Met(Tyr1230/Tyr1234/Tyr1235)Standard:(PartNo.17-470I)Twovialsofphospho-Met(Tyr1230/Tyr1234/Tyr1235)standard,lyophilized.
10.PlateCovers:Twoplatecovers.

Detectionmethod

ChromogenicColorimetric

StorageandShippingInformation
StorageConditions	1yearat4°C

Applications
Application	Thephospho-Met(Tyr1230/Tyr1234/Tyr1235)colorimetricSTARELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtoquantifyspecificlevelsofsignalingtargetsindenaturedcellextracts.
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	Met
EntrezGeneNumber	NM_000245.2
EntrezGeneSummary	Theproto-oncogeneMETproductisthehepatocytegrowthfactorreceptorandencodestyrosine-kinaseactivity.Theprimarysinglechainprecursorproteinispost-translationallycleavedtoproducethealphaandbetasubunits,whicharedisulfidelinkedtoformthematurereceptor.VariousmutationsintheMETgeneareassociatedwithpapillaryrenalcarcinoma.
GeneSymbol	MET HGFR AUTS9 c-Met RCCP2
Modifications	Phosphorylation
UniProtNumber	P08581
UniProtSummary	FUNCTION:SwissProt:P08581#Receptorforhepatocytegrowthfactorandscatterfactor.Hasatyrosine-proteinkinaseactivity. SIZE:1390aminoacids;155527Da SUBUNIT:Heterodimerformedofanalphachain(50kDa)andabetachain(145kDa)whicharedisulfidelinked.BindsPLXNB1andGRB2.InteractswithSPSB1,SPSB2andSPSB4(Bysimilarity).InteractswithINPP5D/SHIP1.WhenphosphorylatedatTyr-1356,interactswithINPPL1/SHIP2.InteractswithRANBP9andRANBP10. SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. DISEASE:SwissProt:P08581#ActivationofMETafterrearrangementwiththeTPRgeneproducesanoncogenicprotein.&DefectsinMETmaybeassociatedwithgastriccancer.&DefectsinMETareacauseofhepatocellularcarcinoma(HCC)[MIM:114550].&DefectsinMETareacauseofhereditarypapillaryrenalcarcinoma(HPRC)[MIM:605074];alsoknownaspapillaryrenalcellcarcinoma2(RCCP2).HPRCisaformofinheritedkidneycancercharacterizedbyapredispositiontodevelopmultiple,bilateralpapillaryrenaltumors.Thepatternofinheritanceisconsistentwithautosomaldominanttransmissionwithreducedpenetrance.&GeneticvariationsinMETmaybeassociatedwithsusceptibilitytoautismtype1B(AUTS1B)[MIM:608636].Autismisaneurodevelopmentaldisordercharacterizedbydisturbanceinlanguage,perceptionandsocialization.Thedisorderisclassicallydefinedbyatriadoflimitedorabsentverbalcommunication,alackofreciprocalsocialinteractionorresponsiveness,andrestricted,stereotypical,andritualizedpatternsofinterestsandbehavior. SIMILARITY:SwissProt:P08581##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.&Contains3IPT/TIGdomains.&Contains1proteinkinasedomain.&Contains1Semadomain.

PhysicochemicalInformation
Sensitivity	Sensitivity:0.78Units/mL. RangeofDetection:1.6to50Units/mL

Dimensions

MaterialsInformation

MaterialsInformation

品牌介绍

Millipore常用产品报价单

产品名	货号	公司分类	商城分类	美金价
Millipore/475855-1R神奇滤布密理博Millipore现货促销	475855-1R	Life Science Research	膜	93.50
Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit	17-701	Life Science Research	定量试剂盒	653.00
Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set	03-110	Life Science Research	抗体套装	433.00
Millipore/CBL468F \| Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays	CBL468F	Antibodies and Assays	功能性抗体	298.00
Millipore/ECM460 \| αVβ3/PECAM Investigator Kit/ECM460/1 kit	ECM460	Antibodies and Assays	夹心法ELISA	477.00