Millipore/17-475 | Phospho-p53 (Ser15) STAR ELISA Kit/17-475/96 assays,Millipore,,Millipore,密理博,merckmillipore,Millipore公司代理生物计划密理博产品目录价格,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/17-475 | Phospho-p53 (Ser15) STAR ELISA Kit/17-475/96 assays

产品编号：17-475

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	17-475
BrandFamily	Upstate
TradeName	STAR Upstate
Description	Phospho-p53(Ser15)STARELISAKit
BackgroundInformation	I.TESTPRINCIPLE ThecolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.Thep53plateiscoatedwithaspecificmousemonoclonalp53captureantibodyonthemicrowellsofthe96-wellclearplate.Samplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingp53antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanyunboundnon-specificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-p53(Ser15)antibodytodetectthecapturedp53ontheplatewellthatisphosphorylatedonSer15.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Thisallowsforasensitiveenzymaticdetectionofthesample. Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtogenerateastandardcurveforphosphorylatedp53(Ser15)measurement. II.p53BACKGROUND p53,agenethatismutatedinapproximatelyhalfofallhumancancers,haslongbeenregardedastheguardianofthegenomebyregulatinggenescontrollingcellcycleprogression,DNArepairandapoptosis.Inresponsetocellularstress,suchasDNAdamage,hypoxia,orrADIation,p53isactivatedandinturnittranscriptionallyactivatestheexpressionofparticulargenes,includingthecyclin-dependentkinaseinhibitorp21,andtogetherwithp19ARF,inducesexpressionofp21Cip1,tocausecellcyclearrest.Alternatively,p53canworkviaapoptosisasawayofeliminatingirreparablydamagedcells. Inactivationorlossofp53hasbeenassociatedwithderegulationofthecellcycleandDNAreplication,inefficientDNArepair,lossofcellularapoptoticresponsesandultimatelythedevelopmentofvarioushumancancers.Thep53polypeptidecontainsthreedistinctregions.Theamino-terminal83aminoacidsofp53containsthetransactivationdomain,aswellastheregioninvolvedintranscription-independentgrowthsuppression,thecentralsequence-specificDNAbindingregion,andthecarboxy-terminalregioncontainstheDNA-bindingdomain.ActivationandstABIlizationofp53isregulatedbyphosphorylationandpossIBLyacetylation
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettorsandtipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	CapturePlatepre-coatedwithanti-p53antibody:(PartNo.17-475A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. Anti-phospho-p53(Ser15)detectionantibody:(PartNo.17-475B)Onebottle(11mL)ofanti-phospho-p53(Ser15)detectionantibodycontainingsodiumazide,readytouse. ELISADiluent:(PartNo.17-475C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse. 25XELISAWashBuffer:(PartNo.17-475D)Onebottle(50mL)of25XELISAWashBuffer. Anti-RabbitIgGHRPconjugate:(PartNo.17-475E)Onevial(125μL)of100Xanti-rabbitHRPconjugate HRPDiluent:(PartNo.17-475F)Onebottle(25mL)ofHRPDiluent. TMBSolution:(PartNo.17-475G)Onebottle(25mL)ofstabilizedtetramethylbenzidine(TMB),readytouse. StopSolution:(PartNo.17-475H)Onebottle(25mL)ofstopsolution,readytouse. Phospho-p53(Ser15)Standard:(PartNo.17-475I)Twovialsofphosphorylatedp53(Ser15)standard,lyophilized. PlateCovers:Twoplatecovers
Detectionmethod	Colorimetric

ProductInformation

Components

CapturePlatepre-coatedwithanti-p53antibody:(PartNo.17-475A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
Anti-phospho-p53(Ser15)detectionantibody:(PartNo.17-475B)Onebottle(11mL)ofanti-phospho-p53(Ser15)detectionantibodycontainingsodiumazide,readytouse.
ELISADiluent:(PartNo.17-475C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
25XELISAWashBuffer:(PartNo.17-475D)Onebottle(50mL)of25XELISAWashBuffer.
Anti-RabbitIgGHRPconjugate:(PartNo.17-475E)Onevial(125μL)of100Xanti-rabbitHRPconjugate
HRPDiluent:(PartNo.17-475F)Onebottle(25mL)ofHRPDiluent.
TMBSolution:(PartNo.17-475G)Onebottle(25mL)ofstabilizedtetramethylbenzidine(TMB),readytouse.
StopSolution:(PartNo.17-475H)Onebottle(25mL)ofstopsolution,readytouse.
Phospho-p53(Ser15)Standard:(PartNo.17-475I)Twovialsofphosphorylatedp53(Ser15)standard,lyophilized.
PlateCovers:Twoplatecovers

Detectionmethod

Colorimetric

StorageandShippingInformation
StorageConditions	Maintaintheunopenedkitat2-8°Cuntilexpirationdate. Precautions •Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata. •CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial. •SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed. •TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin. •TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.Thimerosalisapossiblemutagenandshouldbehandledaccordingly.

StorageandShippingInformation

StorageConditions

Maintaintheunopenedkitat2-8°Cuntilexpirationdate.

Precautions
•Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata.
•CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial.
•SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed.
•TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin.
•TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.Thimerosalisapossiblemutagenandshouldbehandledaccordingly.

Applications
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	Phospho-p53(Ser15)
EntrezGeneNumber	NM_000546.3
EntrezGeneSummary	Tumorproteinp53,anuclearprotein,playsanessentialroleintheregulationofcellcycle,specificallyinthetransitionfromG0toG1.Itisfoundinverylowlevelsinnormalcells,however,inavarietyoftransformedcelllines,itisexpressedinhighamounts,andbelievedtocontributetotransformationandmalignancy.p53isaDNA-bindingproteincontainingDNA-binding,oligomerizationandtranscriptionactivationdomains.Itispostulatedtobindasatetramertoap53-bindingsiteandactivateexpressionofdownstreamgenesthatinhibitgrowthand/orinvasion,andthusfunctionasatumorsuppressor.Mutantsofp53thatfrequentlyoccurinanumberofdifferenthumancancersfailtobindtheconsensusDNAbindingsite,andhencecausethelossoftumorsuppressoractivity.AlterationsoftheTP53geneoccurnotonlyassomaticmutationsinhumanmalignancies,butalsoasgermlinemutationsinsomecancer-pronefamilieswithLi-Fraumenisyndrome.
GeneSymbol	TP53 P53 TRP53 p53 LFS1
Modifications	Phosphorylation
UniProtNumber	P04637
UniProtSummary	FUNCTION:SwissProt:P04637#Actsasatumorsuppressorinmanytumortypes;inducesgrowtharrestorapoptosisdependingonthephysiologicalcircumstancesandcelltype.Involvedincellcycleregulationasatrans-activatorthatactstonegativelyregulatecelldivisionbycontrollingasetofgenesrequiredforthisprocess.Oneoftheactivatedgenesisaninhibitorofcyclin-dependentkinases.ApoptosisinductionseemstobemediatedeitherbystimulationofBAXandFASantigenexpression,orbyrepressionofBcl-2expression. COFACTOR:Binds1zincionpersubunit. SIZE:393aminoacids;43653Da SUBUNIT:InteractswithAXIN1.ProbablypartofacomplexconsistingofTP53,HIPK2andAXIN1(Bysimilarity).BindsDNAasahomotetramer.InteractswithhistoneacetyltransferasesEP300andmethyltransferasesHRMT1L2andCARM1,andrecruitsthemtopromoters.Invitro,theinteractionofTP53withcancer-associated/HPV(E6)viralproteinsleadstoubiquitinationanddegradationofTP53givingapossiblemodelforcellgrowthregulation.Thiscomplexformationrequiresanadditionalfactor,E6-AP,whichstablyassociateswithTP53inthepresenceofE6.C-terminusinteractswithTAF1,whenTAF1ispartoftheTFIIDcomplex.InteractswithING4andthisinteractionmaybeindirect.FoundinacomplexwithCABLES1andTP73.InteractswithHIPK1,HIPK2,andP53DINP1.InteractswithWWOX.MayinteractswithHCVcoreprotein.InteractswithUSP7andSYVN1.InteractswithHSP90AB1(Bysimilarity).InteractswithBANP. SUBCELLULARLOCATION:Cytoplasm.Nucleus.Endoplasmicreticulum.Note=InteractionwithBANPpromotesnuclearlocalization. DOMAIN:SwissProt:P04637Thenuclearexportsignalactsasatranscriptionalrepressiondomain. PTM:Acetylated.AcetylationofLys-382byCREBBPenhancestranscriptionalactivity.DeacetylationofLys-382bySIRT1impairsitsabilitytoinduceproapoptoticprogramandmodulatecellsenescence.&PhosphorylationonSerresiduesmediatestranscriptionalactivation.PhosphorylatedbyHIPK1(Bysimilarity).PhosphorylatedonThr-18byVRK1,whichmaypreventtheinteractionwithMDM2.PhosphorylatedonThr-55byTAF1,whichpromotesMDM2-mediateddegradation.PhosphorylatedonSer-46byHIPK2uponUVirradiation.PhosphorylationonSer-46isrequiredforacetylationbyCREBBP.PhosphorylatedonSer-392followingUVbutnotgammairradiation.PhosphorylateduponDNAdamage,probablybyATMorATR.PhosphorylatedonSer-15uponultravioletirradiation;whichisenhancedbyinteractionwithBANP.&DephosphorylatedbyPP2A.SV40smallTantigeninhibitsthedephosphorylationbytheACformofPP2A.&MaybeO-glycosylatedintheC-terminalbasicregion.StudiedinEB-1cellline.&UbiquitinatedbySYVN1,whichleadstoproteasomaldegradation. DISEASE:SwissProt:P04637#TP53isfoundinincreasedamountsinawidevarietyoftransformedcells.TP53isfrequentlymutatedorinactivatedinabout60%ofcancers.&DefectsinTP53areinvolvedinesophagealsquamouscellcarcinoma(ESCC)[MIM:133239].ESCCisatumoroftheesophagus.&DefectsinTP53areacauseofLi-Fraumenisyndrome(LFS)[MIM:151623].LFSisanautosomaldominantfamilialcancersyndromethatinitsclassicformisdefinedbytheexistenceofbothaprobandwithasarcomaandtwootherfirst-degreerelativeswithacancerbyage45years.Inthesefamiliestheaffectedrelativesdevelopadiversesetofmalignanciesatunusuallyearlyages.ThespectrumofcancersinLFSincludesbreastcarcinomas,soft-tissuesarcomas,braintumors,osteosarcoma,leukemiaandadreno-corticalcarcinoma.OtherpossiblecomponenttumorsofLFSaremelanoma,gonadalcelltumorsandcarcinomasofthelung,pancreasandprostate.&DefectsinTP53maybeassociatedwithnasopharyngealcarcinoma[MIM:161550];alsoknownasnasopharyngealcancer.&DefectsinTP53arefoundinBarrettmetaplasia;alsoknownasBarrettesophagus.Itisaconditioninwhichthenormallystratifiedsquamousepitheliumoftheloweresophagusisreplacedbyametaplasticcolumnarepithelium.Theconditiondevelopsasacomplicationinapproximately10%ofpatientswithchronicgastroesophagealrefluxdiseaseandpredisposestothedevelopmentofesophagealadenocarcinoma.&DefectsinTP53areinvolvedinheadandnecksquamouscellcarcinomas(HNSCC)[MIM:275355].&DefectsinTP53areinvolvedinoralsquamouscellcarcinoma(OSCC).Cigarettesmokeisaprimemutagenicagentincanceroftheaerodigestivetract.&DefectsinTP53areacauseoflungcancer[MIM:211980].&DefectsinTP53areacauseofchoroidplexuspapilloma[MIM:260500].Choroidplexuspapillomaisaslow-growingbenigntumorofthechoroidplexusthatofteninvadestheleptomeninges.Inchildrenitisusuallyinalateralventriclebutinadultsitismoreofteninthefourthventricle.Hydrocephalusiscommon,eitherfromobstructionorfromtumorsecretionofcerebrospinalfluid.Ifitundergoesmalignanttransformationitiscalledachoroidplexuscarcinoma.Primarychoroidplexustumorsarerareandusuallyoccurinearlychildhood.&DefectsinTP53areacauseofoneformofhereditaryadrenocorticalcarcinoma(ADCC)[MIM:202300].ADCCisararechildhoodtumor,representingabout0.4%ofchildhoodtumors,withahighincidenceofassociatedtumors.ADCCoccurswithincreasedfrequencyinpatientswiththeBeckwith-Wiedemannsyndrome[MIM:130650]andisacomponenttumorinLi-Fraumenisyndrome[MIM:151623]. SIMILARITY:Belongstothep53family.

PhysicochemicalInformation
Sensitivity	Sensitivity:1units/mL RangeofDetection:1.6to100units/mL

Dimensions

MaterialsInformation

MaterialsInformation

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