Millipore/17-478 | phospho-PRAS40 (Thr246) STAR ELISA Kit/17-478/96 assays,Millipore,,Millipore,密理博,merckmillipore,Millipore公司代理生物计划 millipore超滤膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/17-478 | phospho-PRAS40 (Thr246) STAR ELISA Kit/17-478/96 assays

产品编号：17-478

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	17-478
BrandFamily	Upstate
TradeName	STAR Upstate
Description	phospho-PRAS40(Thr246)STARELISAKit
AlternateNames	SignalTranscductionAssayReactionPRAS40ELISA
BackgroundInformation	I.TESTPRINCIPLE TheUPSTATE®colorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.ThePRAS40plateiscoatedwithaspecificmousemonoclonalPRAS40captureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingPRAS40antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanyunboundnon-specificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-PRAS40(Thr246)antibodytodetectthecapturedPRAS40ontheplatewellthatisphosphorylatedonThr246.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Thisallowsforasensitiveenzymaticdetectionofthesample. Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.ThekitalsoincludesastandardthatisrunasbothapositivecontrolandtogenerateastandardcurveforphosphorylatedPRAS40(Thr246)measurement. II.PRAS40BACKGROUND PRAS40(ProlineRichAktSubstrate,40kDa),alsoknownasAKT1S1,isaubiquitouslyexpressproteinthatisphosphorylatedonresidueThr246viathePI3K/Aktpathway.ItwasoriginallydiscoveredasanAktsubstrateasithastheputativeAktphosphorylationmotifofRxRxxpS/pT2.Thisphosphorylationallowsittobind14-3-3andRaptorofthemTORcomplexmTORC1.PRAS40isknowntobindtoandregulatemTORC1(mTOR,Raptor,mLST8)kinasesactivitythatisactivatedbyinsulindownstreamofPI3KandAktandsubsequentlyphosphorylatesp70S6Kand4EBP1.PRAS40isknowntohaveaputativeTOSmotif(FVMDE)thatallowsitbindtoRaptorofmTORC1intheabsenceofinsulin1.ItisthoughtthatthroughitbindingtotheRaptor,PRAS40inhibitsthekinaseactivityofmTORC1bypreventingtobindtoitssubstratessuchasp70S6Kand4EBP1.
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettorsandtipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	1.CapturePlatepre-coatedwithanti-PRAS40antibody:(PartNo.17-478A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. 2.Anti-phospho-PRAS40(Thr246)detectionantibody:(PartNo.17-478B)Onebottle(11mL)ofanti-phospho-PRAS40(Thr246)detectionantibodycontainingsodiumazide,readytouse. 3.ELISADiluent:(PartNo.17-478C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse. 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-478E)Onevial(125μL)of100Xanti-rabbitHRPconjugate. 6.HRPDiluent:(PartNo.17-478F)Onebottle(25mL)ofHRPDiluent. 7.TMBSolution:(PartNo.17-478G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse 8.StopSolution:(PartNo.17-478H)Onebottle(25mL)ofstopsolution,readytouse. 9.Phospho-PRAS40(Thr246)Standard:(PartNo.17-478I)TwovialsofphosphorylatedPRAS40(Thr246)standard,lyophilized. 10.PlateCovers:Twoplatecovers.
Detectionmethod	Colorimetric

ProductInformation

Components

1.CapturePlatepre-coatedwithanti-PRAS40antibody:(PartNo.17-478A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
2.Anti-phospho-PRAS40(Thr246)detectionantibody:(PartNo.17-478B)Onebottle(11mL)ofanti-phospho-PRAS40(Thr246)detectionantibodycontainingsodiumazide,readytouse.
3.ELISADiluent:(PartNo.17-478C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
5.Anti-RabbitIgGHRPconjugate:(PartNo.17-478E)Onevial(125μL)of100Xanti-rabbitHRPconjugate.
6.HRPDiluent:(PartNo.17-478F)Onebottle(25mL)ofHRPDiluent.
7.TMBSolution:(PartNo.17-478G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse
8.StopSolution:(PartNo.17-478H)Onebottle(25mL)ofstopsolution,readytouse.
9.Phospho-PRAS40(Thr246)Standard:(PartNo.17-478I)TwovialsofphosphorylatedPRAS40(Thr246)standard,lyophilized.
10.PlateCovers:Twoplatecovers.

Detectionmethod

Colorimetric

StorageandShippingInformation
StorageConditions	Maintaintheunopenedkitat2-8°Cuntilexpirationdate. Precautions •Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata. •CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial. •SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed. •TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin. •TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

StorageandShippingInformation

StorageConditions

Maintaintheunopenedkitat2-8°Cuntilexpirationdate.
Precautions
•Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata.
•CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial.
•SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed.
•TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin.
•TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

Applications
Application	Thephospho-PRAS40(Thr246)colorimetricSTARELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtoquantifyspecificlevelsofsignalingtargetsindenaturedcellextracts.
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	phospho-PRAS40(Thr246)
EntrezGeneNumber	NM_001098632.1 NM_001098633.1 NM_032375.3
EntrezGeneSummary	AKT1S1isaproline-richsubstrateofAKT(MIM164730)thatbinds14-3-3protein(seeYWHAH,MIM113508)whenphosphorylated(Kovacinaetal.,2003[PubMed12524439]).[suppliedbyOMIM]
GeneSymbol	AKT1S1 Lobe MGC2865 PRAS40
Modifications	Phosphorylation
UniProtNumber	Q96B36
UniProtSummary	FUNCTION:SwissProt:Q96B36#Mayplayanimportantroleinphosphatidylinositol3-kinase(PI3K)-AKT1survivalsignaling.SubstrateforAKT1phosphorylation,butcanalsobeactivatedbyAKT1-independentmechanisms.Itsroleinsurvivalsignalingpathwaysmaybemodulatedbyoxidativestress.Mayalsoplayaroleinnervegrowthfactor-mediatedneuroprotection. SIZE:256aminoacids;27383Da SUBUNIT:Thephosphorylatedforminteractswith14-3-3. SUBCELLULARLOCATION:Cytoplasm,cytosol(Bysimilarity).Note=Foundinthecytosolicfractionofthebrain(Bysimilarity). TISSUESPECIFICITY:Widelyexpressedwithhighestlevelsofexpressioninliverandheart.Expressedathigherlevelsincancercelllines(e.g.A549andHeLa)thaninnormalcelllines(e.g.HEK293).

PhysicochemicalInformation
Sensitivity	Sensitivity:1unit/mL RangeofDetection:1.6to100units/mL SpeciesReactivity:Human

Dimensions

MaterialsInformation

MaterialsInformation

品牌介绍

Millipore常用产品报价单

产品名	货号	公司分类	商城分类	美金价
Millipore/475855-1R神奇滤布密理博Millipore现货促销	475855-1R	Life Science Research	膜	93.50
Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit	17-701	Life Science Research	定量试剂盒	653.00
Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set	03-110	Life Science Research	抗体套装	433.00
Millipore/CBL468F \| Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays	CBL468F	Antibodies and Assays	功能性抗体	298.00
Millipore/ECM460 \| αVβ3/PECAM Investigator Kit/ECM460/1 kit	ECM460	Antibodies and Assays	夹心法ELISA	477.00