Millipore/17-485 | IκB STAR ELISA Kit/17-485/96 assays,Millipore,,Millipore,密理博,merckmillipore,生命科学Millipore价格优惠进口密理博现货,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/17-485 | IκB STAR ELISA Kit/17-485/96 assays

产品编号：17-485

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	17-485
TradeName	STAR Upstate
Description	IκBSTARELISAKit
BackgroundInformation	TestPrinciple TheUPSTATE®colorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheIkBplateiscoatedwithaspecificmousemonoclonalIkBcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingIkBantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-IkBantibodytodetectthecaptureIkBontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader. Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardlineofdetection. IkBBackground IKKbeta(IkBKinase,IKKb,I-Kappa-Bkinase-beta)isamemberoftheIKKcomplexwhichiscomposedofIKKalpha,IKKbeta,IKKgammaandIKAP.IkBKinase(IKK)complexisacriticalcomponentinNFBregulation.Inamajorityofunstimulatedcells,theNFκBtranscriptionfactorsexistintheirinactiveformandareretainedinthecytoplasmbytheboundinhibitoryIkBproteins.ForNFBtobecomeactivated,itmustfirstdisassociatefromtheinhibitorIκB.ThisoccursviatheserinephosphorylationofIkBonSer32andSer36.Postreceptorstimulation,mostnotablybyTNFα,NIK,aserine/threoninekinase,becomesactivatesandphosphorylatesIKKαandIKKβ.Phosphorylationof2sitesattheactivationloopofIKKbetaisessentialforactivationofIKKbyTNFandIL1.Onceactivated,IKKbetaautophosphorylates,whichinturndecreasesIKKactivityandpreventsprolongedactivationoftheinflammatoryresponse.Additionally,IKKbetaactivitycanalsoberegulatedbyMEKK-1.IKKthenbindstoandphosphorylatesIkBαonthetwoserineresidues.ThisphosphorylationmarksIkBforubiquinationanddestructionbytheproteosome.ThisfreetheNFkBcomplexandallowsittotranslocatestothenucleus.
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	1.CapturePlatepre-coatedwithanti-I_κBantibody:(PartNo.17-485A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. 2.Anti-I_κBdetectionantibody:(PartNo.17-485B)Onebottle(11mL)ofanti-I_κBdetectionantibodycontainingsodiumazide,readytouse. 3.ELISADiluent:(PartNo.17-485C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse. 4.25XELISAWashBuffer:(PartNo.17-485D)Onebottle(50mL)of25XELISAWashBuffer. 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-485E)Onevial(125μL)of100Xanti-rabbitHRPconjugate. 6.HRPDiluent:(PartNo.17-485F)Onebottle(25mL)ofHRPDiluent. 7.TMBSolution:(PartNo.17-485G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse. 8.StopSolution:(PartNo.17-485H)Onebottle(25mL)ofstopsolution,readytouse. 9.I_κBStandard:(PartNo.17-485I)TwovialsofI_κBstandard,lyophilized. 10.PlateCovers:Twoplatecovers.
Detectionmethod	Colorimetric

ProductInformation

Components

1.CapturePlatepre-coatedwithanti-I_κBantibody:(PartNo.17-485A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
2.Anti-I_κBdetectionantibody:(PartNo.17-485B)Onebottle(11mL)ofanti-I_κBdetectionantibodycontainingsodiumazide,readytouse.
3.ELISADiluent:(PartNo.17-485C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
4.25XELISAWashBuffer:(PartNo.17-485D)Onebottle(50mL)of25XELISAWashBuffer.
5.Anti-RabbitIgGHRPconjugate:(PartNo.17-485E)Onevial(125μL)of100Xanti-rabbitHRPconjugate.
6.HRPDiluent:(PartNo.17-485F)Onebottle(25mL)ofHRPDiluent.
7.TMBSolution:(PartNo.17-485G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
8.StopSolution:(PartNo.17-485H)Onebottle(25mL)ofstopsolution,readytouse.
9.I_κBStandard:(PartNo.17-485I)TwovialsofI_κBstandard,lyophilized.
10.PlateCovers:Twoplatecovers.

Detectionmethod

Colorimetric

StorageandShippingInformation
StorageConditions	1yearat4°Cfromdateofshipment

Applications
Application	TheIκBcolorimetricSTARELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtoquantifyspecificlevelsofsignalingtargetsindenaturedcellextracts.
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	IκB
EntrezGeneNumber	NM_020529.1
EntrezGeneSummary	NFKB1(MIM164011)orNFKB2(MIM164012)isboundtoREL(MIM164910),RELA(MIM164014),orRELB(MIM604758)toformtheNFKBcomplex.TheNFKBcomplexisinhibitedbyI-kappa-Bproteins(NFKBIAorNFKBIB,MIM604495),whichinactivateNF-kappa-Bbytrappingitinthecytoplasm.PhosphorylationofserineresiduesontheI-kappa-Bproteinsbykinases(IKBKA,MIM600664,orIKBKB,MIM603258)marksthemfordestructionviatheubiquitinationpathway,therebyallowingactivationoftheNF-kappa-Bcomplex.ActivatedNFKBcomplextranslocatesintothenucleusandbindsDNAatkappa-B-bindingmotifssuchas5-primeGGGRNNYYCC3-primeor5-primeHGGARNYYCC3-prime(whereHisA,C,orT;RisanAorGpurine;andYisaCorTpyrimidine).[suppliedbyOMIM]
GeneSymbol	NFKBIA IKB-alpha IKBA I-kappa-B-alpha MAD-3 MAD3 IkappaBalpha NFKBI
UniProtNumber	P25963
UniProtSummary	FUNCTION:SwissProt:P25963#InhibitsNF-kappa-Bbycomplexingwithandtrappingitinthecytoplasm.MaybeinvolvedinregulationoftranscriptionalresponsestoNF-kappa-B,includingcelladhesion,immuneandproinflammatoryresponses,apoptosis,differentiationandgrowth.Controlledbysequentialserine-phosphorylation,ubiquitinationanddegradation.Tyrosine-phosphorylationcouldonlyleadtodissociationfromNF-kappa-B. SIZE:317aminoacids;35609Da SUBUNIT:Interactswithp65(RELA).InteractswithNKIRAS1andNKIRAS2.InteractswithHBVproteinX. SUBCELLULARLOCATION:Cytoplasm. PTM:Phosphorylated;disablesinhibitionofNF-kappa-BDNA-bindingactivity.&Ubiquitinated;subsequenttostimulus-dependentphosphorylationonserines,polyubiquitinationtargetstheproteinforrapiddegradationviatheubiquitinsystem. DISEASE:SwissProt:P25963#DefectsinNFKBIAareacauseofautosomaldominantanhidroticectodermaldysplasiawithimmunodeficiency(AD-EDA-ID)[MIM:164008].Ectodermaldysplasias(EDs)constituteaheterogeneousgroupofdevelopmentaldisordersaffectingtissuesofectodermalorigin.EDsarecharacterizedbyabnormaldevelopmentoftwoormoreectodermalstructuressuchashair,teeth,nailsandsweatglands,withorwithoutanyadditionalclinicalsign.Eachcombinationofclinicalfeaturesrepresentsadifferenttypeofectodermaldysplasia. SIMILARITY:SwissProt:P25963##BelongstotheNF-kappa-Binhibitorfamily.&Contains5ANKrepeats.

PhysicochemicalInformation
Sensitivity	Sensitivity:62.5pg/mL. RangeofDetection:62.5to4000pg/mL

Dimensions

MaterialsInformation

MaterialsInformation

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