Description | |
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CatalogueNumber | 17-608 |
BrandFamily | Upstate |
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Description | ChIPAb+HDAC1-ChIPValidatedAntibodyandPrimerSet |
Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HDAC1setincludestheHDAC1antibody,thenegativecontrolantibodysupernatant(mouseIgG),andqPCRprimersflankinganSp1bindingsiteinhumanp21(WAF1/CIP1/CDKN1A)promoter,amplifyinga105basepairPCRproduct.TheHDAC1andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHDAC1associatedchromatin. |
ProductInformation | |
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Format | CultureSupernatant |
Control |
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Presentation | Anti-HDAC1(mousemonoclonalIgG1,supernatant).Onevialcontaining40μLofculturesupernatantwith0.05%sodiumazide.Storeat-20°C. NegativeChIPControlSupernatant.Onevialcontaining40uLofmouseIgGcontainingsupernatantwith0.05%sodiumazide.Storeat-20°C. ChIPPrimersp21.Onevialcontaining75μLof5μMofeachprimerspecificforaregionofthehumanp21(WAF1/CIP1/CDKN1A)promoter.Storeat-20°C. P21Forward:CCCACAGCAGAGGAGAAAGAA P21Reverse:CTGGAAATCTCTGCCCAGACA |
StorageandShippingInformation | |
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StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt |
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Millipore常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |