商品介绍
| Description | |
|---|---|
| CatalogueNumber | 14-828 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | HDAC4,activerecombinantprotein |
| Overview | RecominanthumanHDAC4(a.a.627-end)withN-terminalGST-tag |
| BackgroundInformation | Histonedeacetylationactsatagforepigeneticrepressionandplaysanimportantroleintranscriptionalregulation,cellcycleprogressionanddevelopmentalevents.HDAC4isahistonedeacetylasewhichdeacetylateslysinesonthecorehistones.HDAC4accumulatesinthenucleiofmyotubes,suggestinginvolvementinmuscledifferentiation.HDAC4doesnotbindDNAdirectlybutinteractswiththemyocyteenhancerfactorsMef2a,Mef2c,andMef2d.HDAC4alsointeractswiththebreastcancer-associatedtranscriptionalrepressorPLU-1/JARID1B,theNr2c1/TR2nuclearreceptor,aswellasthe14-3-3chaperoneprotein,inaphosphorylationdependentmanner.ItalsoformspartofamultiproteincomplexwithRbAp48andHDAC3. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | 50μgofrecombinantprotienin50mMTris-HCl,pH7.0,150mMNaCl,0.1mMEDTA,1mMdithiothreitol,20%glycerol.Frozensolution. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storeinaliquotsat-70°C.Avoidfreeze/thawcycles.Formaximumrecoveryofproduct,centrifugeoriginalvialpriortoremovingcap. |
| Applications | |
|---|---|
| Application | RecominanthumanHDAC4(a.a.627-end)withN-terminalGST-tag. |
| KeyApplications |
|
| ApplicationNotes | HDACdeacetylaseassays |
| BIOLOGicalInformation | |
|---|---|
| Concentration | 0.2mg/ml |
| Purity | Purity>90%bySDS-PAGEandCoomassiebluestaining. |
| Source | ExpressedbybaculovirusinSf9insectcells. |
| SpecificActivity | [Varieswithlot.ForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme.]LotR0801D0003:2.7U/mg,whereoneunitofHDAC4activityisdefinedas1nmolofassaysubstrate(HDACassaykit,catalog#17-356)deacetylatedperminuteat37°C. |
| EntrezGeneNumber | |
| GeneSymbol |
|
| PurificationMethod | Purifiedusingglutathione-agarose.GSTportionwascleavedbyPreScission™protease. |
| UniProtNumber | |
| MolecularWeight | 49kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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