Description | |
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CatalogueNumber | F1903 |
BrandFamily | Chemicon® |
TradeName |
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Description | Fibronectin40kDaαChymotrypticFragment(Heparin-bindingregion),Humanpurified |
Overview | Heparin-binding40kDafragmentofhumanplasmafibronectinpurifiedfromchymotrypticdigestofhumanplasmafibronectin.ThispreparationcontainstheCS-1heparin-bindingregionoffibronectin.Heparin-bindingfragmentpromotesCytoskeletonorganizationinintegrinsignaling.Thispreparationshowsonemajorbandat40kDainSDS-PAGEwithnohighermolecularweightspeciespresent.Smallerproteolyticfragmentsmaybepresent. HumansourcedrawmaterialusedintheproductionofthisproducttestednegativeforhepatitisBvirus,hepatitisCvirus(HCV),humanimmunodeficiencyvirustype-1(HIV-1)andtype-2(HIV-2),humanT-celllymphotropicvirus(HTLV-1andHTLV-2)andTreponemapallidum.Handleasifpotentiallyinfectious. |
ProductInformation | |
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Presentation | Purifiedbygelatin-Sepharose™andheparin-Sepharose™columnchromatography.Lyophilizedfromsodiumcitratebufferedsaline,20mM,NaCl150mM,pH7.4.Reconstitutewithdistilledwater. |
StorageandShippingInformation | |
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StorageConditions | Maintainlyophilizedmaterialat-20°Cforuptooneyearfromdateofreceipt.Afterreconstitution,maintainat-20°Cforuptosixmonthsinundilutedaliquots.Avoidrepeatedfreeze/thawcycles. |
Applications | |
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KeyApplications |
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ApplicationNotes | ELISA Affinitychromatography PossIBLyformolecularmappingoffibronectinfunction Cellattachmentandintegrinsignalingassays Optimalworkingdilutionsmustbedeterminedbytheenduser. |
BIOLOGicalInformation | |
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Concentration | 1mg/mL |
Source | Human |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined. |
GeneSymbol |
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UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains. |
StemCellType |
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PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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Millipore常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |