Millipore/FC010-100MG | Human Plasma Fibronectin Purified Protein/FC010-100MG/100 mg,Millipore,,Millipore,密理博,merckmillipore,正品Millipore生物试剂1 millipore PTFE膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/FC010-100MG | Human Plasma Fibronectin Purified Protein/FC010-100MG/100 mg

产品编号：FC010-100MG

市场价：¥38520.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1926.00

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	FC010-100MG
Replaces	FC010-250MG
BrandFamily	Chemicon®
TradeName	Chemicon
Description	HumanPlasmaFibronectinPurifiedProtein
Overview	Humanfibronectin(HFN)issuitableforuseasanattachmentfactorinthepropagationofcellsinvitrowhenusedtocoatcellculturesurfaces,includingplasticware,glassware,andmicrocarrierbeads.

ProductInformation
Presentation	Lyophilizedfrom150mMNaCl,10mMsodiumphosphate,pH7.5,containingnopreservatives.

StorageandShippingInformation
StorageConditions	Maintainlyophilizedmaterialat-20°Cforupto12monthsfromdateofreceipt.Storethereconstitutedmaterialat2º-8°Cforupto6months.

Applications
KeyApplications	CellCulture
ApplicationNotes	Immunochemicalstandard Cellattachmentandproliferationassaysonhumanendothelialcells,humankeratinocytesandhumandermalfibroblasts SuggestedProcedureforCoatingCellCultureware 1.DeterminetheamountofhFNneededtocoatculturevesselsbymultiplyingthetotalsurfacearea(cm2)bythedesiredconcentration(mg/mL)ofhFN.Recommendedamountis2-10mg/cm2. 2.ReconstitutetheentirevialofhFNto1mg/mLusingdistilledordeionizedwaterat37ºC.AddthelyophilizedhFNslowlywithconstantstirring,andavoidexcessivefoaming.Breakupanyclumpsthatmightformagainstthesideofthecontainerwithaglassrodorspatula.SterilefilterthehFNsolutiontoremoveanyremaininginsolublematerial.Storethereconstitutedmaterialat2º-8°Cforupto6months.Forworkingdilutions,dilutethematerialtothedesiredconcentrationwithphysiologicalbuffer.FordeterminingfinalproteinconcentrationsbyOD280,theExtinctioncoefficient=1.3[(mg/mL)xcm]-1). 3.Wetthesurfaceofeachculturevesseltobecoatedwithaminimumamountofsterilebalancedsaltsolution(serumandproteinfree)requiredtocovertheentirearea. 4.IntroducetheproperCO2atmosphere,ifrequired. 5.AddthecalculatedamountofhFNtoeachculturevesselandstoretheremaininghFNat2º-8ºCasindicatedbelow. 6.AllowhFNtoadsorbtothesurfaceofthevesselfor5-20minutes. 7.Removeresidualbalancedsaltsolutionbeforeproceedingwithstandardcellcultureprocedures. Note:hFNislyophilizedfrombufferedsalineandtheweightofthematerialisnotonlyoftheproteinitself,butincludestheprotein+salts.Thematerialmaynotbehomogeneouswithregardtoprotein/saltratio.

Applications

KeyApplications

CellCulture

ApplicationNotes

Immunochemicalstandard

Cellattachmentandproliferationassaysonhumanendothelialcells,humankeratinocytesandhumandermalfibroblasts

SuggestedProcedureforCoatingCellCultureware

1.DeterminetheamountofhFNneededtocoatculturevesselsbymultiplyingthetotalsurfacearea(cm2)bythedesiredconcentration(mg/mL)ofhFN.Recommendedamountis2-10mg/cm2.

2.Reconstitutetheentirevial*ofhFNto1mg/mLusingdistilledordeionizedwaterat37ºC.AddthelyophilizedhFNslowlywithconstantstirring,andavoidexcessivefoaming.Breakupanyclumpsthatmightformagainstthesideofthecontainerwithaglassrodorspatula.SterilefilterthehFNsolutiontoremoveanyremaininginsolublematerial.Storethereconstitutedmaterialat2º-8°Cforupto6months.Forworkingdilutions,dilutethematerialtothedesiredconcentrationwithphysiologicalbuffer.FordeterminingfinalproteinconcentrationsbyOD280,theExtinctioncoefficient=1.3[(mg/mL)xcm]-1).

3.Wetthesurfaceofeachculturevesseltobecoatedwithaminimumamountofsterilebalancedsaltsolution(serumandproteinfree)requiredtocovertheentirearea.

4.IntroducetheproperCO2atmosphere,ifrequired.

5.AddthecalculatedamountofhFNtoeachculturevesselandstoretheremaininghFNat2º-8ºCasindicatedbelow.

6.AllowhFNtoadsorbtothesurfaceofthevesselfor5-20minutes.

7.Removeresidualbalancedsaltsolutionbeforeproceedingwithstandardcellcultureprocedures.

*Note:hFNislyophilizedfrombufferedsalineandtheweightofthematerialisnotonlyoftheproteinitself,butincludestheprotein+salts.Thematerialmaynotbehomogeneouswithregardtoprotein/saltratio.

BIOLOGicalInformation
Purity	Approximately95%,asdeterminedbySDS-PAGE.Adoublebandof220kDaispresentunderreducedconditions.HFNispurifiedbyaffinitychromatographyongelatinagarose,followedbychromatographyonheparin-agarose.
Source	Humandonorplasma.
EntrezGeneNumber	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
EntrezGeneSummary	Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
GeneSymbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProtNumber	P02751
UniProtSummary	FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.
StemCellType	HumanEmbryonicStemCells MesenchymalStemCells NeuralStemCells HematopoieticStemCells EpithelialCells PancreaticStemCells InducedPluripotentStemCells

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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