产品名称：Millipore/ECM101 | Millicoat™ Human Fibronectin Coated Strips (96-Wells)/ECM101/1 plate96 wells
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ECM101 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Millicoat™HumanFibronectinCoatedStrips(96-Wells) |
| Overview | Celladhesionplaysamajorroleincellularcommunicationandregulation,andisoffundamentalimportanceinthedevelopmentandmaintenanceoftissues.Scientistsarecontinuallyexaminingtheadhesionandmigrationofmanydiversecelltypesonvariousextracellularmatrix(ECM)componentproteins.Millicoat™CellAdhesionStripsareprovidedas12x8-wellremovablestripsinaplateframeforconvenienceandflexibilityindesigningassays.ThewellsinrowsA-GhavebeencoatedwithHumanFibronectin.RowHofeachstripiscoatedwithBSAwhichservesasanegativeassaycontrol.Cellsareseededontothecoatedsubstrate.Subsequently,adherentcellsarefixedandstained.RelativeattachmentisdeterminedusingabsorbancereADIngs. |
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| ProductInformation | |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maybestoredat2-8°Cinthefoilpouchforatleast3months.Unusedstripsmaybeplacedbackinthepouchforstorage.Ensurethatthedesiccantremainsinthepouch,andthatthepouchissecurelyclosed. |
| Applications | |
|---|---|
| Application | MillicoatCellAdhesionStripsareprovidedas12x8-wellremovablestripsina96-wellplateframeforconvenience&flexibilityindesigningassays. |
| KeyApplications |
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| ApplicationNotes | PROCEDURE: NOTE:Optimalassaytimingandperformancemayvaryfordifferentcelllinesbutgenerallycanbeobtainedusingsubconfluentcellculturesintheassaydescribedbelow.Subconfluentculturescanbeachievedbysplittingcells1to2dayspriortoperformingtheassay. 1.Rehydratethestripswith200mLofPBSperwellforatleast15minutesatroomtemperature.RemovethePBSfromtherehydratedstrips. 2.PrepareasinglecellsUSPension,preferablyusinganon-enzymaticdissociationbuffer.Optimumcelldensitymaybedeterminedbytitrationofthecells.Acommonstartingrangeisbetween1x10E05to1x10E07cells/mL. 3.Add100mLofthedilutedcellsuspensiontoeachwell.Incubatetheplateat37°Cfor1hourinaCO2incubator.Gentlywashtheplate2-3timeswithPBScontainingCa2+/Mg2+(200mL/well). 4.Add100mLof0.2%crystalvioletin10%ethanoltoeachwell.Incubatefor5minutesatroomtemperature.Removethestainfromthewells.Gentlywashtheplate3timeswithPBS(300mL/well). 5.Add100mLofSolubilizationBuffer(A50/50mixtureof0.1MNaH2PO4,pH4.5and50%ethanol)toeachwell.Allowstripstoincubateandgentlyshakeatroomtemperatureuntilthecell-boundstainiscompletelysolubilized;approximately5minutes. 6.Determinetheabsorbanceat540-570nmonamicroplatereader. |
| BIOLOGicalInformation | |
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| SpeciesReactivity |
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| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains. |
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| PhysicochemicalInformation |
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| MaterialsInformation |
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Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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