Millipore/ECM201 | QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric/ECM201/1 kitSufficient for 12 a,Millipore,,Millipore,密理博,merckmillipore,Millipore试剂产品信息密理博抗体品牌,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/ECM201 | QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric/ECM201/1 kitSufficient for 12 a

产品编号：ECM201

市场价：¥9980.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$499.00

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	ECM201
TradeName	QCM Chemicon
Description	QCM3µmEndothelialCellMigrationAssay-Fibronectin,Fluorometric
Overview	Alsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere Introduction Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration. Millipore"s3μmQCM™EndothelialCellMigrationAssay–Fibronectin,FluorometricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement. Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample.
MaterialsRequiredbutNotDelivered	1.Endothelialcells(forexample:HUVECcells),andcellculturemedium(forexample:EGM-2). 2.Serum-freemedium,suchasserum-freeEGM-2containing0.5%BSA. 3.SterilePBSorHBSStowashcells. 4.Distilledwater 5.(Optional)Chemoattractantorpharmacologicalagentforadditiontoculturemedium. 6.Lowspeedcentrifugeandtubesforcellharvesting 7.CO₂incubatorappropriateforsubjectcells 8.Hemocytometerorothermeansofcountingcells. 9.Trypanblueorequivalentviabilitystain 10.Fluorescenceplatereader.

ProductInformation
Components	1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples. 2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls. 3.4XCellLysisBuffer-One16mLbottle 4.CyQuantGRDye®:Onevial75μL 5.24-WellCellCultureTray 6.96-WellStainQuantitationPlate 7.Forceps:1pair 8.Accutase(celldetachmentsolution):One100mLbottle
Detectionmethod	ColorimetricFluorescent

ProductInformation

Components

1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
3.4XCellLysisBuffer-One16mLbottle
4.CyQuantGRDye®:Onevial75μL
5.24-WellCellCultureTray
6.96-WellStainQuantitationPlate
7.Forceps:1pair
8.Accutase(celldetachmentsolution):One100mLbottle

Detectionmethod

ColorimetricFluorescent

StorageandShippingInformation
StorageConditions	•TheECM201-1kitcomponentsshouldbestoredat2°to8°Cupuntiltheexpirationdateprovidedonthelabels.DONOTFREEZE. •Theaccompanying100mlbottleofAccutasealsoincludedinthiskitshouldbestoredat-20°C.

Applications
Application	ThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.
ApplicationNotes	TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricassayisintendedforresearchuseonly;notfordiagnosticapplications. Eachkitprovidessufficientmaterialsfortheevaluationof12samples.

Applications

Application

ThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.

ApplicationNotes

TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricassayisintendedforresearchuseonly;notfordiagnosticapplications.
Eachkitprovidessufficientmaterialsfortheevaluationof12samples.

BIOLOGicalInformation
SpeciesReactivity	Vertebrates

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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