商品介绍
| Description | |
|---|---|
| CatalogueNumber | ECM205 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Millicoat™ECMScreeningKit,1ea.ECM101-ECM105 |
| Overview | Celladhesionplaysamajorroleincellularcommunicationandregulation,andisoffundamentalimportanceinthedevelopmentandmaintenanceoftissues.Scientistsarecontinuallyexaminingtheadhesionandmigrationofmanydiversecelltypesonvariousextracellularmatrix(ECM)componentproteins.Millicoat™CellAdhesionStripsareprovidedas128-wellremovablestripsinaplateframeforconvenienceandflexibilityindesigningassays.ThewellsinrowsA-GhavebeencoatedwithahumanECMprotein.RowHofeachstripiscoatedwithBSAwhichservesasanegativeassaycontrol.TheMillicoatTMscreeningkitcontainsindividual96-wellplatesforfibronectin,vitronectin,laminin,collagenIandcollagenIV.Cellsareseededontothecoatedsubstrate.Subsequently,adherentcellsarefixedandstained.RelativeattachmentisdeterminedusingabsorbancereADIngs. |
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| ProductInformation | |
|---|---|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maybestoredat2-8°Cinthefoilpouchforatleast3months.Unusedstripsmaybeplacedbackinthepouchforstorage.Ensurethatthedesiccantremainsinthepouch,andthatthepouchissecurelyclosed. |
| Applications | |
|---|---|
| Application | TheMillicoatTMscreeningkitcontainsindividual96-wellplatesforfibronectin,vitronectin,laminin,collagenI&collagenIV. |
| KeyApplications |
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| ApplicationNotes | PROCEDURE: NOTE:Optimalassayperformanceisobtainedusingsubconfluentcellcultures.Thiscanbeachievedbysplittingthecells1to2dayspriortoperformingtheassay. 1.Rehydratethestripswith200mLofPBSperwellforatleast15minutesatroomtemperature.RemovethePBSfromtherehydatedstrips. 2.PrepareasinglecellsUSPension,preferablyusinganon-enzymaticdissociationbuffer.Optimumcelldensitymaybedeterminedbytitrationofthecells.Acommonstartingrangeisbetween1x10E05to1x10E07cells/mL. 3.Add100mLofthedilutedcellsuspensiontoeachwell.Incubatetheplateat37°Cfor1hourinaCO2incubator.Gentlywashtheplate3timeswithPBScontainingCa2+/Mg2+(200mL/well). 4.Add100mL/wellof0.2%crystalvioletin10%ethanoltoeachwell.Incubatefor5minutesatroomtemperature.Removethestainfromthewells.Gentlywashtheplate3timeswithPBS(300mL/well). 5.Add100mLofSolubilizationBuffer(A50/50mixtureof0.1MNaH2PO,pH4.5and50%ethanol)toeachwell.Allowstripstoincubateandgentlyshakeatroomtemperatureuntilthecell-boundstainiscompletelysolubilized;approximately5minutes. 6.Determinetheabsorbancesat540-570nmonamicroplatereader. |
| BIOLOGicalInformation | |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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