Description | |
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CatalogueNumber | ECM580 |
Replaces | ECM500 |
BrandFamily | Chemicon® |
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Description | QCMHaptotaxisCellMigrationAssay-Fibronectin,24-well,colorimetric |
Overview | Alsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere Introduction TheCHEMICONQCM™HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricisidealforthestudyofepithelialandfibroblastcellhaptotaxis.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The8μmporesizeofthisassay"sBoydenchambersisnotappropriateforthestudyoflymphocytemigration. •Eachkitprovidessufficientmaterialsfortheevaluationof12samples. •Pleaserefertotheproductinsertforassaydetails. TheCHEMICONQCM™HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications. |
MaterialsRequiredbutNotDelivered | 1.PrecisionPipettes:sufficientforaliquotingcells. 2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Chemicon"sready-to-usenon-mammaliandetachmentsolution,Accutase™(Cat.No.SCR005)canalsobeused. 3.Tissueculturegrowthmediumappropriateforsubjectcells,suchasDMEMcontaining5%FBS. 4.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer. 5.SterilePBSorHBSStowashcells. 6.Distilledwater 7.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium. 8.Lowspeedcentrifugeandtubesforcellharvesting. 9.CO2incubatorappropriateforsubjectcells. 10.Hemocytometerorothermeansofcountingcells. 11.TrypanblueorequivalentviABIlitystain. 12.Microplatereader(540-570nmdetection)orspectrophotometer. 13.Sterilecellculturehood 14.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane. 15.Shaker |
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Components |
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Detectionmethod | Chromogenic |
StorageandShippingInformation | |
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StorageConditions | Storeat4°Cforuptooneyearafterdateofpurchase. |
Applications | |
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Application | TheQCMHaptotaxisCellMigrationAssay,24-wellplate-Fibronectin,Colorimetricisidealforthestudyofepithelial&fibroblastcellhaptotaxis. |
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