商品介绍
| Description | |
|---|---|
| CatalogueNumber | NS220 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | NeuriteOutgrowthAssayKit(3µm) |
| Overview | Application CHEMICON"sNS220NeuriteOutgrowthAssayKit(3µm)utilizesmicroporoustissuecultureinserttechnologyfromMilliporeandisbasedontheuseofMillicellcellcultureinserts(chambers)containingapermeablemembranewith3-μmporesatthebase.TheMillicellinsertsutilizedinthiskitareappropriateforcellsprojectingneuritesofupto3µmdiameter,e.g.N1E-115cells[Abeetal.,2003],DorsalRootGangliaandSchwanncells[Windebanketal.,1985].Theinsertsaredesignedtofitintoareceivervessel,whichwheninusecontainsdifferentiationmediaincontactwiththebottomoftheinsertmembrane.Thepermeablemembranesallowfordiscriminationbetweenneuritesandcellbodies,asprojectingneuriteswillpasseasilythroughtheporesbutcellbodieswillnot.Therefore,byinducingneuritestotraversethesemembranepores,apurifiedpopulationofneuritesislocatedontheundersideofthemembrane,distaltothesurfaceonwhichneuralcellbodiesaredeposited. Theassayisasimple,efficient,andversatilesystemforthequantitativedeterminationofcompoundsthatinfluenceneuriteformationandrepulsion.Withthissystem,itispossIBLetoscreennumerousBIOLOGicalandpharmacologicalagentssimultaneously,directlyevaluateadhesionandguidancereceptorfunctionsresponsibleforneuriteextensionandrepulsion,aswellasanalyzegenefunctionintransfectedcells.Themicroporousfilterallowsforbiochemicalseparationandpurificationofneuritesandcellbodiesfordetailedmolecularanalysisofproteinexpression,signaltransductionprocessesandidentificationofdrugtargetsthatregulateneuriteoutgrowthorretractionprocesses.ReagentsandmaterialssuppliedintheNS220NeuriteOutgrowthAssayKitaresufficientfor12tests. Note:the3µmporesizeinsertsinthiskitareunsuitableforusewithPC12cells.DuetotherelativelysmalldiameterofPC12cellbodies[Abeetal.,2003],someofthesecellsmaypassthough3µmporesandobscuretheresults.Chemicon"sNS225NeuriteOutgrowthAssayKit(1µm)hasbeenspecificallydesignedforandvalidatedwithPC12cells. |
| MaterialsRequiredbutNotDelivered | 1.Desiredextracellularmatrixmembranecoatingprotein,e.g.,bovinecollagenIormouselaminin. 2.Tissuecultureinstruments/supplies(including37°Cincubator,growthmedia,flasks/plates,detachmentbuffer,etc.)forcelltypeofinterest. 3.Differentiationmedia/factorsforcelltypeofinterest(e.g.,nervegrowthfactor,serum-freemedia). 4.1Xphosphatebufferedsaline(PBS)withCa2+/Mg2+. 5.Invertedmicroscopeforneuritevisualizationonmembraneinserts. 6.Parafilmandspectrophotometerforneuritestainextractionandquantification. 7.Proteinlysisbuffer(optional) |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Whenstoredat2-8°C,thekitcomponentsarestableuptotheexpirationdate.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate. |
| Applications | |
|---|---|
| Application | TheNS220NeuriteOutgrowthAssayKit(3µm)isbasedontheuseofMillicellcellcultureinserts(chambers)containingapermeablemembranewith3-μmporesatthebase. |
| KeyApplications |
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| BiologicalInformation | |
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Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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