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主营：分子类，蛋白类，抗体类，生化类试剂
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℡ 4000-520-616
Millipore/MAB351R | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6/MAB351R/100 µg
产品编号：MAB351R
市  场 价：¥8020.00
场      地：美国(厂家直采)
产品分类： 抗体类>一抗>功能性抗体>
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：$401.00
品      牌： Millipore
公      司：Millipore
公司分类：
Millipore/MAB351R | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6/MAB351R/100 µg
商品介绍
Description
CatalogueNumberMAB351R
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-GlutamateDecarboxylaseAntibody,65kDaisoform,cloneGAD-6
AlternateNames
  • GAD65
BackgroundInformationGutamicaciddecarboxylase(GAD;E.C.4.1.1.15)istheenzymeresponsIBLefortheconversionofglutamicacidtogamma-aminobutyricacid(GABA),themajorinhibitorytransmitterinhigherbrainregions,andputativeparacrinehormoneinpancreaticislets.TwomolecularformsofGAD(65kDaand67kDa,64%aaidentitybetweenforms)arehighlyconservedandbothformsareexpressedintheCNS,pancreaticisletcells,testis,oviductandovary.Theisoformsareregionallydistributedcytoplasmicallyinthebrainsofratsandmice(Sheikh,S.etal.1999).GAD65isanampiphilic,membrane-anchoredprotein(585aa),encodedonhumanchromosome10,andisresponsibleforvesicularGABAproduction.GAD67iscytoplasmic(594aa.),encodedonchromosome2,andseemstoberesponsibleforsignificantcytoplasmicGABAproduction.GADexpressionchangesduringneuraldevelopmentinratspinalcord.GAD65isexpressedtransientlyincommissuralaxonsaroundE13butisdownregulatedthenextdaywhileGAD67expressionincreasesmostlyinthesomataofthoseneurons(Phelps,P.etal.1999).Inmatureratpancreas,GAD65andGAD67appeartobedifferentiallylocalized,GAD65primarilyininsulin-containingbetacellsandGAD67inglucagon-containing(A)cells(Li,L.etal.1995).GAD67expressionseemstobeparticularlyplasticandcanchangeinresponsetoexperimentalmanipulation(forexampleneuronalstimulationortransection)ordiseaseprogressionandemergentdisorderslikeschizophrenia(Volketal.,2000).ColocalizationofthetwoGADisoformsalsoshowschangesinGAD65/GAD67distributionscorrelatedwithcertaindiseasestatessuchasIDDMandSMS.
ProductInformation
FormatPurified
Control
  • Braintissue
PresentationLyophilized.Dissolvecontentsofvialin100µLofsterile,distilledwater.Thisresultsinafinalantibodyconcentrationof1mg/mlin10mMpotassiumphosphate,70mMsodiumchloride,pH7.4containingnopreservatives.
StorageandShippingInformation
StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Applications
ApplicationAnti-GlutamateDecarboxylaseAntibody,65kDaisoform,cloneGAD-6isanantibodyagainstGlutamateDecarboxylaseforuseinIH&WB.
KeyApplications
  • Immunohistochemistry
  • WesternBlotting
ApplicationNotesImmunohistochemistry:(<=1=""μg/ml)=""optimal=""working=""dilutions=""must=""be=""determined=""by=""end=""user.="">



ImmunohystochemicalStainingProcedures

ThefollowingprocedurewasdevelopedtolocalizeGADinratbrainsectionsofcerebellum.Performallstepsatroomtemperatureunlessotherwiseindicated.Wherenormalserumisindicated,usenormalserumfromthesamespeciesasthesourceofthesecondaryantibody.Thisprocedurerepresentssuggestedguidelinesfortheuseofanti-GAD.Fixationregimen,antibodyconcentrations,andincubationconditionsforagivenexperimentalsystemshouldbedeterminedempirically.

1.Perfuseratswith100mMphosphatebuffer,pH7.4,containing1%paraformaldehyde,0.34%L-lysine,and0.05%sodiumm-periodate(1%PLP).

2.Postfixbrainsin1%PLPfor1-2hours.Longerfixationtimesmayreducelabelingintensity.

3.Transferbrainsto100mMphosphatebuffercontaining30%sucrose,andgentlyagitateonashakerplatformat+4°Cfor48-60hours.

4.Usingaslidingmicrotome,cut30mmsectionsoffrozencerebellum.Asthesectionsarecut,collecttheminavialofcold100mMphosphatebuffer.

5.Incubatesectionsinphosphate-bufferedsaline(PBS)containing1.5%normalserumand0.2%TritonX-100for30minutes.

6.Onashakerplatform,incubatesectionswithanti-GAD(dilutedinPBScontaining1.5%normalserumand0.2%TritonX-100toafinalantibodyconcentrationof1mg/ml)for12-36hoursat+4°C.

7.Onashakerplatform,rinsesectionseighttimes,10-15minutesperrinse,inPBS.

8.Detectwithastandardsecondaryantibodydetectionsystem(Hsuetal.,1981;Falini&Taylor,1983;Harlow&Lane,1988;Taylor,1978).

9.Mountsections,dehydrate,andapplycoverslips.
BIOLOGicalInformation
ImmunogenPurifiedratbrainGAD.
CloneGAD-6
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityRecognizesthelowermolecularweightisoformofthetwoGADisoformsidentifiedinbrain(Gottlieb,etal.,1986;Chang&Gottlieb,1988).Thismonclonalantibodycanbeusedforimmunohistochemicallocalizationinbrainorpancreas.Anti-GADhasalsobeenusedtolabelpurifiedGADonWesternblots(Chang&Gottlieb,1988).
IsotypeIgG2a
SpeciesReactivity
  • Human
  • Rat
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesoneofseveralformsofglutamicaciddecarboxylase,identifiedasamajorautoantigenininsulin-dependentdiabetes.Theenzymeencodedisresponsibleforcatalyzingtheproductionofgamma-aminobutyricacidfromL-glutamicacid.ApathogenicroleforthisenzymehasbeenidentifiedinthehumanpancreassinceithasbeenidentifiedasanautoantibodyandanautoreactiveTcelltargetininsulin-dependentdiabetes.Thisgenemayalsoplayaroleinthestiffmansyndrome.
GeneSymbol
  • GAD2
  • MGC161607
  • GAD65
  • MGC161605
  • GAD-65
  • EC4.1.1.15
PurificationMethodAmmoniumsulfateprecipitationandDEAE-cellulosechromatography
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q05329#CatalyzestheproductionofGABA.
COFACTOR:Pyridoxalphosphate.
SIZE:585aminoacids;65411Da
SUBUNIT:Homodimer(Bysimilarity).
SUBCELLULARLOCATION:Cytoplasm,cytosol.Cytoplasmicvesicle.Celljunction,synapse,presynapticcellmembrane;Lipid-anchor.Golgiapparatusmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Associatedtocytoplasmicvesicles.Inneurons,cytosolicleafletofGolgimembranesandpresynapticclusters.
PTM:Phosphorylated;whichdoesnotaffectkineticparametersorsubcellularlocation.&Palmitoylated;whichisrequiredforpresynapticclustering.
SIMILARITY:SwissProt:Q05329##BelongstothegroupIIdecarboxylasefamily.
MolecularWeight65kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
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