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CatalogueNumber | S7804 |
BrandFamily | Chemicon® |
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Description | CpGWIZ®E-CadherinAmplificationKit |
Overview | E-cadherinisacelladhesionproteinthatsuppressestumorcellinvasionandmetastasisintumormodels.OnmechanismregulatingtheexpressionoftheE-cadheringeneismethylationofthepromoterregion.TheCpGWIZ®E-cahedrinAmplificationKitisusedfordeterminingthemethylationofstatusoftheE-cadherinpromoterbymethylation-specificPCR(MSP).Thekitcontainsprimerstargetedtoregionsofthepromoterwherethesequencesaremostdivergentafterbisulfitetreatment.PCRparametershavebeenidentifiedsothatallprimersetsinthekitamplifyunderthesameconditions.ControlgenomicDNAsamples(methylatedandunmethylated)forE-cadherinarealsoincluded. Methylation-specificPCR(MSP)isanewtechnologyforsensitivedetectionofabnormalgenemethylationutilizingsmallamountsofDNA(Herman,1996).ThisprocessemploysaninitialbisulfitereactiontomodifytheDNA,followedbyPCRamplificationwithspecificprimersdesignedtodistinguishmethylatedfromunmethylatedDNA. |
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EntrezGeneSummary | Thisgeneisaclassicalcadherinfromthecadherinsuperfamily.Theencodedproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Mutationsinthisgenearecorrelatedwithgastric,breast,colorectal,thyroidandovariancancer.Lossoffunctionisthoughttocontributetoprogressionincancerbyincreasingproliferation,invasion,and/ormetastasis.Theectodomainofthisproteinmediatesbacterialadhesiontomammaliancellsandthecytoplasmicdomainisrequiredforinternalization.Identifiedtranscriptvariantsarisefrommutationatconsensussplicesites. |
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UniProtSummary | FUNCTION:SwissProt:P12830#E-Cad/CTF2promotesnon-amyloidogenicdegradationofAbetaprecursors.HasastronginhibitoryeffectonAPPC99andC83production. SIZE:882aminoacids;97456Da SUBUNIT:Homodimer;disulfide-linked.Interactsdirectly,viathecytoplasmicdomain,withCTNNB1orJUPtoformthePSEN1/cadherin/cateninadhesioncomplexwhichconnectstotheactinskeletonthroughtheactinbindingofalpha-catenin.InteractionwithPSEN1,cleavesCDH1resultinginthedisassociationofcadherin-basedadherensjunctions(CAJs).InteractswithAJAP1,CTNND1andDLG7. SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein.Note=ColocalizeswithDLG7atsitesofcell-cellcontactinintestinalepithelialcells.AnchoredtoactinMICROFILamentsthroughassociationwithalpha-,beta-andgamma-catenin.Sequentialproteolysisinducedbyapoptosisorcalciuminflux,resultsintranslocationfromsitesofcell-cellcontacttothecytoplasm. TISSUESPECIFICITY:Non-neuralepithelialtissues. PTM:Duringapoptosisorwithcalciuminflux,cleavedbyamembrane-boundmetalloproteinase(ADAM10),PS1/gamma-secretaseandcaspase-3toproducefragmentsofabout38kDa(E-CAD/CTF1),33kDa(E-CAD/CTF2)and29kDa(E-CAD/CTF3),respectively.Processingbythemetalloproteinase,inducedbycalciuminflux,causesdisruptionofcell-celladhesionandthesubsequentreleaseofbeta-cateninintothecytoplasm.Theresidualmembrane-tetheredcleavageproductisrapidlydegradedviaanintracellularproteolyticpathway.Cleavagebycaspase-3releasesthecytoplasmictailresultingindisintegrationoftheactinmicrofilamentsystem.Thegamma-secretase-mediatedcleavagepromotesdisaaaemblyofadherensjunctions. DISEASE:"SwissProt:P12830#DefectsinCDH1areinvolvedindysfunctionofthecell-celladhesionsystem,triggeringcancerinvasion(gastric,breast,ovary,endometriumandthyroid)andmetastasis.&DefectsinCDH1areacauseofhereditarydiffusegastriccancer(HDGC)[MIM:137215].&DefectsinCDH1areacauseofsusceptibilitytoendometrialcancer[MIM:608089]." SIMILARITY:SwissProt:P12830##Contains5cadherindomains. |
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Millipore常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |