Millipore/2750 | BrdU Cell Proliferation Kit/2750/200 assays,Millipore,,Millipore,密理博,merckmillipore,Millipore代理，产品目录 millipore一级代理,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/2750 | BrdU Cell Proliferation Kit/2750/200 assays

产品编号：2750

市场价：¥6780.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$339.00

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	2750
BrandFamily	Chemicon®
TradeName	Chemicon
Description	BrdUCellProliferationKit
Overview	TestPrinciple WithCHEMICON®"sBrdUCellProliferationAssayKit,mousemonoclonalantibodyisusedtodetecttheBrdUinasample.Afteradditionofgoatanti-mouseIgG-peroxidaseconjugatedsecondaryantibody,substrateandstopsolution,theamountofBrdUisdetermined.ThehighertheOD,thehighertheBrdUconcentrationinthesample.
MaterialsRequiredbutNotDelivered	1.Pipettors&tipscapableofaccuratelymeasuring2-1000μL 2.Multi-channelorrepeatingPipettes 3.Washbottleormultichanneldispensorforwashing 4.2000mLgraduatedcylinder 5.PBS(137mMNaCl,2.7mMKCl,4.3mMNa2HPO4-7H2O,1.4mMKH2PO4) 6.DeionizedordistilledH2O. 7.96-wellmicroplatereaderwith450-540nmor450-595nmdualwavelengthor450nmsinglewavelengthfilter 8.Tissueculturemicroplate(96wellculturedish) 9.Sterilereagenttroughs 10.Microsyringefilter(0.2μm) 11.Syringe
BackgroundInformation	Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA.

ProductInformation
Components	BrdUReagent:(PartNo.2750a)One15μLvialofa500XsolutionofBrdU. FixingSolution:(PartNo.2750b)One40mLbottle. PredilutedBrdUDetectionAntibody:(PartNo.2750c)One20mLvialofananti-BrdUsolution. StopSolution:(PartNo.2750d):One25mLbottleof2.5Nsulfuricacidsolution. Goatanti-MouseIgG,Peroxidaselabeled:(PartNo.2750e)One15μLvialofa2000Xsolution. ConjugateDiluent:(PartNo.2750f)One25mLbottle. Substrate:(PartNo.2750g)One25mLbottle,readytouseTMB. 50XPlateWashConcentrate:(PartNo.2750h)One90mLbottleofbufferedTriswithsurfactant.
Detectionmethod	Colorimetric

ProductInformation

Components

BrdUReagent:(PartNo.2750a)One15μLvialofa500XsolutionofBrdU.
FixingSolution:(PartNo.2750b)One40mLbottle.
PredilutedBrdUDetectionAntibody:(PartNo.2750c)One20mLvialofananti-BrdUsolution.
StopSolution:(PartNo.2750d):One25mLbottleof2.5Nsulfuricacidsolution.
Goatanti-MouseIgG,Peroxidaselabeled:(PartNo.2750e)One15μLvialofa2000Xsolution.
ConjugateDiluent:(PartNo.2750f)One25mLbottle.
Substrate:(PartNo.2750g)One25mLbottle,readytouseTMB.
50XPlateWashConcentrate:(PartNo.2750h)One90mLbottleofbufferedTriswithsurfactant.

Detectionmethod

Colorimetric

StorageandShippingInformation
StorageConditions	Maintainthekitat2°to8°Cundersterileconditionsforupto6monthsafterdateofreceipt. Beforefirstuse:removetheFixativeSolutionandplaceatroomtemperatureforatleast4hourspriortouse.Precipitatesthatmayoccurduringcoldstorageshouldgobackintosolution.

Applications
Application	TheBrdUCellProliferationAssayKitisusedtodetecttheBrdUinasample.
KeyApplications	ELISA
ApplicationNotes	TheCHEMICON®BrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells. ForResearchUseOnly.Notforuseindiagnosticprocedures.

BIOLOGicalInformation
SpeciesReactivity	Mouse Human Rat Pig Horse Rabbit GuineaPig Hamster Non-humanPrimate Canine

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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