Millipore/2752 | BrdU Cell Proliferation Kit/2752/1000 assays,Millipore,,Millipore,密理博,merckmillipore,millipore醋酸纤维素膜 millipore CA膜,聚丙烯膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/2752 | BrdU Cell Proliferation Kit/2752/1000 assays

产品编号：2752

市场价：¥8500.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$425.00

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	2752
BrandFamily	Chemicon®
TradeName	Chemicon
Description	BrdUCellProliferationKit
Overview	TheCHEMICONBrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells. TestPrinciple WithinCHEMICON"sBrdUCellProliferationAssayKit,mousemonoclonalantibodyisusedtodetecttheBrdUinasample.Afteradditionofgoatanti-mouseIgG-peroxidaseconjugatedsecondaryantibody,substrateandstopsolution,theamountofBrdUisdetermined.ThehighertheOD,thehighertheBrdUconcentrationinthesample. ForResearchUseOnly.Notforuseindiagnosticprocedures.
BackgroundInformation	Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA.
MaterialsRequiredbutNotDelivered	1.Pipettors&tipscapableofaccuratelymeasuring2-1000μL 2.Multi-channelorrepeatingPipettes 3.Washbottleormultichanneldispensorforwashing 4.2000mLgraduatedcylinder 5.PBS(137mMNaCl,2.7mMKCl,4.3mMNa2HPO4-7H2O,1.4mMKH2PO4) 6.DeionizedordistilledH2O. 7.96-wellmicroplatereaderwith450-540nmor450-595nmdualwavelengthor450nmsinglewavelengthfilter 8.Tissueculturemicroplate(96wellculturedish) 9.Sterilereagenttroughs 10.Microsyringefilter(0.2mm) 11.Syringe

ProductInformation
Components	BrdUReagent:(PartNo.2752a)One100μLvialofa500XsolutionofBrdU. FixingSolution:(PartNo.2752b)One200mLbottle. PredilutedBrdUDetectionAntibody:(PartNo.2752c)One100mLvialofanti-BrdUinsolution. StopSolution:(PartNo.2752d):One100mLbottleof2.5Nsulfuricacidsolution. Goatanti-MouseIgG,Peroxidaselabeled:(PartNo.2752e)One60μLvialofa2000Xsolution. ConjugateDiluent:(PartNo.2752f)One100mLbottle. Substrate:(PartNo.2752g)One100mLbottle,readytouseTMB. 50XPlateWashConcentrate:(PartNo.2752h)One125mLbottleofbufferedTriswithsurfactant.
Detectionmethod	Colorimetric

ProductInformation

Components

BrdUReagent:(PartNo.2752a)One100μLvialofa500XsolutionofBrdU.
FixingSolution:(PartNo.2752b)One200mLbottle.
PredilutedBrdUDetectionAntibody:(PartNo.2752c)One100mLvialofanti-BrdUinsolution.
StopSolution:(PartNo.2752d):One100mLbottleof2.5Nsulfuricacidsolution.
Goatanti-MouseIgG,Peroxidaselabeled:(PartNo.2752e)One60μLvialofa2000Xsolution.
ConjugateDiluent:(PartNo.2752f)One100mLbottle.
Substrate:(PartNo.2752g)One100mLbottle,readytouseTMB.
50XPlateWashConcentrate:(PartNo.2752h)One125mLbottleofbufferedTriswithsurfactant.

Detectionmethod

Colorimetric

StorageandShippingInformation
StorageConditions	Maintaintheunopenedkitat2-8°Cuntiltheexpirationdateindicatedonthelabel. Beforefirstuse:removetheFixativeSolutionandplaceatroomtemperatureforatleast4hourspriortouse.Precipitatesthatmayoccurduringcoldstorageshouldgobackintosolution.

Applications
Application	TheBrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells.
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Mouse Human Rat Pig Horse Rabbit GuineaPig Hamster Non-humanPrimate Canine

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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