Millipore/2760 | BrdU IHC Kit/2760/50 ea,Millipore,,Millipore,密理博,merckmillipore,品牌试剂实验Millipore代理 millipore超滤膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/2760 | BrdU IHC Kit/2760/50 ea

产品编号：2760

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	2760
BrandFamily	Chemicon®
TradeName	Chemicon
Description	BrdUIHCKit
Overview	TestPrinciple CHEMICON®"sBrdUImmunohistochemistryKitisanon-isotopicimmuno-histochemistrystainingkitforthedetectionandlocalizationofBrdUincorporatedintonewlysynthesizedDNAofproliferatingcells.VisualstainingofthecellsisachievedbyusingabiotinylatedmonoclonalantibodytoBrdUfollowedbystreptavidin-HRPconjugateandDABsubstrate.
BackgroundInformation	Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA.
MaterialsRequiredbutNotDelivered	1.QuenchingSolution:Hydrogenperoxide(30%)solutionforquenchingendogenousperoxidaseactivity. 2.Phosphatebufferedsaline(PBS)solution. 3.Distilledwater. 4.Ethylalcohol 5.Xylene 6.Coverslips 7.Bromodeoxyuridine(BrdU) 8.Methanol

ProductInformation
Components	4XTrypsinEnzymeConcentrate:(PartNo.2760a)One3mLbottle. Tryspinisonlyrequiredifyouareusingformalinfixedtissues.Ifthetissuesarefixedinalcohol,trypsindigestionisnotnecessary. TrypsinDilutionSolution*:(PartNo.2760b)One12mLbottle. DenaturingSolution:(PartNo.2760c)One6mLbottle. BlockingBuffer:(PartNo.2760d)One6mLbottle. DetectorAntibody(Biotinylatedandready-to-use):(PartNo.2760e)One6mLbottle. Streptavidin-HRPConjugate(ready-to-use):(PartNo.2760f)One6mLbottle. SubstrateReactionBuffer:(PartNo.2760g)One6mLbottle. DABConcentrate:(PartNo.2760h)One0.3mL. HematoxylinCounterstain:(PartNo.2760i)One6mLbottle. MountingMedia:(PartNo.2760j)One6mLbottle. 5ControlSlides:IntestinaltissuefrommouseinjectedwithBrdU.1Positivelystained(PartNo.2760k)and4unstained(PartNo.2760l).

ProductInformation

Components

4XTrypsinEnzymeConcentrate*:(PartNo.2760a)One3mLbottle.

*Tryspinisonlyrequiredifyouareusingformalinfixedtissues.Ifthetissuesarefixedinalcohol,trypsindigestionisnotnecessary.
TrypsinDilutionSolution*:(PartNo.2760b)One12mLbottle.
DenaturingSolution:(PartNo.2760c)One6mLbottle.
BlockingBuffer:(PartNo.2760d)One6mLbottle.
DetectorAntibody(Biotinylatedandready-to-use):(PartNo.2760e)One6mLbottle.
Streptavidin-HRPConjugate(ready-to-use):(PartNo.2760f)One6mLbottle.
SubstrateReactionBuffer:(PartNo.2760g)One6mLbottle.
DABConcentrate:(PartNo.2760h)One0.3mL.
HematoxylinCounterstain:(PartNo.2760i)One6mLbottle.
MountingMedia:(PartNo.2760j)One6mLbottle.
5ControlSlides:IntestinaltissuefrommouseinjectedwithBrdU.1Positivelystained(PartNo.2760k)and4unstained(PartNo.2760l).

StorageandShippingInformation
StorageConditions	Maintaintheunopenedkitat-20°Cforupto6monthsafterdateofreceipt.Afteropeningthekititmaybestoredat2°to8°Cfor4-5days.Forlong-termstorageitisrecommendedthatyoualiquoteachcomponentandfreezeat-20°Cforupto3monthsafteropening.Isitrecommendedthatyouwearlatexorrubbergloveswhenrunningthiskit,especiallyavoidcontactwiththeDABreagent.

Applications
KeyApplications	Immunohistochemistry(Paraffin)
ApplicationNotes	TheCHEMICON®KitisdesignedforinvitrodetectionofBrdUinproliferatingcells.Thematerialinthiskitissufficienttorun50slides.Theaveragetestareaisdefinedasacirclearoundthetissuewithanapproximatediameterof2centimeters. ForResearchUseOnly.Notforuseindiagnosticprocedures.

BIOLOGicalInformation

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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