Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays,Millipore,,Millipore,密理博,merckmillipore,进口Millipore品牌清关代理 millipore聚偏氟乙烯膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays

产品编号：APT115

市场价：¥14200.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$710.00

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	APT115
BrandFamily	Chemicon®
TradeName	Apo-BrdU Chemicon
Description	Apo-BrdU™DetectionKit
Overview	TheCHEMICONAPO-BRDU™KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyflowcytometry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding;positiveandnegativecontrolcellsforassessingreagentperformance;washing,reaction,andrinsingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),andfluoresceinlabeledanti-BrdUantibodyforlabelingDNAbreaksandPropidiumIodide/RNaseAsolutionforcounterstainingthetotalDNA.
MaterialsRequiredbutNotDelivered	1.FlowCytometer 2.DistilledWater 3.1%(w/v)paraformaldehyde(methanolfree)inPBS 4.70%(v/v)ethanol 5.37°CWaterBath 6.IceBucket 7.12x75mmflowcytometrytesttubes 8.Micro-pipettor

ProductInformation
Components	TheAPO-BRDU™Kitisshippedinonecontainerandconsistsoftwopackages.Onepackageisshippedatambienttemperatureandshouldbestoredat2-8°Cuponarrival.Theotherpackageisstyrofoamcontainingfrozenicepacksandthereagentcontentsshouldbestoredat-20°Cuponarrival.Note:Uponarrivalstorethereagentsattheappropriatetemperatures. Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess60cellsUSPensionsincluding5mLpositiveand5mLnegativecontrolcellsuspensionsofapproximately1x106cellspermLin70%(v/v)ethanol.Thecontrolcellsarederivedfromahumanlymphomacelllineandhavebeenfixedasdescribedonpage6. PositiveControlCells,Browncap NegativeControlCells,Whitecap WashBuffer,Bluecap ReactionBuffer,Greencap TdTEnzyme,Yellowcap Br-dUTP,Violetcap RinsingBuffer,Redcap Fluorescein~PRB-1mAb,Orangecap PI/RNaseStainingBuffer,Amberbottle
Detectionmethod	Fluorescent

ProductInformation

Components

TheAPO-BRDU™Kitisshippedinonecontainerandconsistsoftwopackages.Onepackageisshippedatambienttemperatureandshouldbestoredat2-8°Cuponarrival.Theotherpackageisstyrofoamcontainingfrozenicepacksandthereagentcontentsshouldbestoredat-20°Cuponarrival.Note:Uponarrivalstorethereagentsattheappropriatetemperatures.
Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess60cellsUSPensionsincluding5mLpositiveand5mLnegativecontrolcellsuspensionsofapproximately1x106cellspermLin70%(v/v)ethanol.Thecontrolcellsarederivedfromahumanlymphomacelllineandhavebeenfixedasdescribedonpage6.
PositiveControlCells,Browncap
NegativeControlCells,Whitecap
WashBuffer,Bluecap
ReactionBuffer,Greencap
TdTEnzyme,Yellowcap
Br-dUTP,Violetcap
RinsingBuffer,Redcap
Fluorescein~PRB-1mAb,Orangecap
PI/RNaseStainingBuffer,Amberbottle

Detectionmethod

Fluorescent

StorageandShippingInformation
StorageConditions	PrecautionsandWarnings 1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures. 2.Positiveandnegativecontrolcellscontain70%(v/v)ethanolasapreservative;washandreactionbuffercontainsodiumcacodylate(dimethylarsinic)asabuffer;rinsingandPI/RNasestainingbuffercontain0.05%(w/v)sodiumazideasapreservative.Thesematerialsareharmfulifswallowed;avoidskincontact,washimmediatelywithwater.SeeMaterialSafetyDataSheets. 3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.UponwarmingtheTdTenzymesolution,centrifugethetubefor30secondstoforcealltheliquidtothebottomofthetube.

StorageandShippingInformation

StorageConditions

PrecautionsandWarnings

1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures.

2.Positiveandnegativecontrolcellscontain70%(v/v)ethanolasapreservative;washandreactionbuffercontainsodiumcacodylate(dimethylarsinic)asabuffer;rinsingandPI/RNasestainingbuffercontain0.05%(w/v)sodiumazideasapreservative.Thesematerialsareharmfulifswallowed;avoidskincontact,washimmediatelywithwater.SeeMaterialSafetyDataSheets.

3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.UponwarmingtheTdTenzymesolution,centrifugethetubefor30secondstoforcealltheliquidtothebottomofthetube.

Applications
Application	Thekitmeasuresapoptosisincells.
KeyApplications	FlowCytometry

BIOLOGicalInformation
SpeciesReactivity	All

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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