Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays,Millipore,,Millipore,密理博,merckmillipore,Millipore公司代理生物计划密理博授权代理商，正品,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays

产品编号：APT116

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	APT116
BrandFamily	Chemicon®
TradeName	Apo-BrdU Chemicon
Description	Apo-BRDUIHCKit
Overview	TheCHEMICONAPO-BRDU-IHC™KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyimmunohistochemistry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding:positiveandnegativecontrolslidesforassessingreagentperformance;reactionandblockingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),biotin-labeledanti-BrdUantibodyforlabelingDNAstrandbreaks,HRP-streptavidinconjugate,DAN,H2O2/Ureatabletsforcolorgeneration,andmethylgreensolutionforcounterstainingthecells.
MaterialsRequiredbutNotDelivered	1.Xylene-100% 2.Ethanol-100%,90%,80%,70%v/vwithdH2O 3.Methanol-100% 4.HydrogenPeroxide-30% 5.PBSorTris-bufferedsaline(1XTBS,20mMTrispH7.6,140mMNaCl) 6.10mMTris,pH8.0 7.1mMMgSO4in1XTBS(foruseingeneratingpositivecontrol) 8.DNaseI(foruseingeneratingpositivecontrol) 9.1-4%FormaldehydeinPBS,pH7.4 10.DistilledWater 11.Coplinjarswithslideholders 12.Beakerforrinsingslides 13.Slidehumidifyingchamber 14.Glasscoverslips 15.Mountingmedia 16.Microscope 17.Micro-pipettorwithdisposabletips 18.Microcentrifugetubes 19.Parafilm®(RegisteredtrademarkofAmericanNationalCanCompany) 20.Absorbentwipes 21.Crushedice

ProductInformation
Components	Positive/NegativeControlSlides,Naturalbox Note:2slides,eachcontainingamixturepositiveandnegativecontrolcells. BlockingBuffer,Whitecap H2O2/UreaTablets,Ambervial ProteinaseK,Pinkcap DABTablets,Ambervial TdTEnzyme,Yellowcap Br-Dutp,Violetcap 200XStreptavidin-HRPConjugate,Blackcap 5XReactionBuffer,Greencap Biotin-labeledBrdUmAb,Orangecap MethylGreensolution,Naturalcap, TheAPO-BRDU-IHC™Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival. Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed. Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.
Detectionmethod	Chromogenic

ProductInformation

Components

Positive/NegativeControlSlides,Naturalbox
Note:2slides,eachcontainingamixturepositiveandnegativecontrolcells.
BlockingBuffer,Whitecap
H2O2/UreaTablets,Ambervial
ProteinaseK,Pinkcap
DABTablets,Ambervial
TdTEnzyme,Yellowcap
Br-Dutp,Violetcap
200XStreptavidin-HRPConjugate,Blackcap
5XReactionBuffer,Greencap
Biotin-labeledBrdUmAb,Orangecap
MethylGreensolution,Naturalcap,
TheAPO-BRDU-IHC™Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival.
Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.
Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	PrecautionsandWarnings: 1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures. 2.Afterinitialdefrosting,storethe5XReactionBufferandBiotin-labeledanti-BrdUantibodyat2-8ºC,andtheMethylGreensolutionatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed. 3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution. 4.The5XReactionbufferandBr-dUTPsolutioncontaincacodylicacidasabuffer.TheBiotin-labeledantibodycontains0.05%sodiumazideasapreservative.TheDABtabletscontaindiaminobenzidine.Thesematerialsareharmfulifswallowed.Avoidskincontactwithanyofthesecomponents;washimmediatelywithwater. 5.Uponwarmingthecomponents,centrifugethetubesfor30secondstoforcealltheliquidtothebottomofthetube. 6.Gloves,labcoatandprotectiveeyewearshouldbewornwhilestaining.

StorageandShippingInformation

StorageConditions

PrecautionsandWarnings:

1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures.

2.Afterinitialdefrosting,storethe5XReactionBufferandBiotin-labeledanti-BrdUantibodyat2-8ºC,andtheMethylGreensolutionatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.

3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.

4.The5XReactionbufferandBr-dUTPsolutioncontaincacodylicacidasabuffer.TheBiotin-labeledantibodycontains0.05%sodiumazideasapreservative.TheDABtabletscontaindiaminobenzidine.Thesematerialsareharmfulifswallowed.Avoidskincontactwithanyofthesecomponents;washimmediatelywithwater.

5.Uponwarmingthecomponents,centrifugethetubesfor30secondstoforcealltheliquidtothebottomofthetube.

6.Gloves,labcoatandprotectiveeyewearshouldbewornwhilestaining.

Applications
Application	TheAPO-BRDU-IHCKitisatwocolorstainingmethodforlabelingDNAbreaks&totalcellularDNAtodetectapoptoticcellsbyIHC.
KeyApplications	Immunohistochemistry

BIOLOGicalInformation
SpeciesReactivity	All

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

品牌介绍

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