产品编号：CBL202-K
市 场 价：¥121690.00
场 地：美国(厂家直采)
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商品介绍
| Description | |
|---|---|
| CatalogueNumber | CBL202-K |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | VimentinMsxHuMg |
| Overview | ThisproductisintendedforusebyIVDmanufacturers. Contactcustomerserviceforassistance:oem.reagents@emdMillipore.com |
| AlternateNames |
|
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | ProteinAPurifiedimmunoglobulinpresentedaslyophilizedmaterial.Reconstitutewith1mldistilledwater.FinalsolutionisPBS,pH7.4,containing0.5%BSAand0.09%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat2–8°Cforupto12monthsfromdateofreceipt. |
| Applications | |
|---|---|
| Application | ThisAnti-VimentinAntibody,cloneVIM3B4isvalidatedforuseinELISA,IF,IH(P),WBforthedetectionofVimentin. |
| KeyApplications |
|
| ApplicationNotes | Westernblot Immunohistochemistry:1:2000;Frozenandparaffin-embeddedtissuesectionswithaminimumofaonehourincubation(longerforparaffin);proteasepretreatmentisrecommendedforparaffin-embeddedsections. Immunofluorescence ELISA Optimalworkingdilutionsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Vimentinpurifiedfrombovinelens |
| Clone | VIM3B4 |
| Concentration | 0.05mg/mL |
| Host | Mouse |
| Specificity | Highlyspecificfortheintermediatefilamentproteinvimentinwhichispresentinallcellsofmesenchymalorigin.Thisantibodyispositivewithcellsofmesenchymalderivation,includingendothelialcellsandcertainsmoothmusclecellsofthevasculartract,fibroblasts,connectivetissue,alltypesofbloodcells,includingthymocytes,interstitialandtesticularSertolicells,ovarianfolliclecells.Itcanalsobeusedtodetectcoexpressionofvimentinincombinationwithotherintermediatefilamentproteins.VIM3B4hasturnedouttobethemostavidmonoclonalantibodytovimentin.Tumorsspecificallydetected:sarcoma(includingmyosarcoma),lymphoma,melanoma.ThebindingregionofmonoclonalantibodyVIM3B4hasbeencharacterizedbyBohnetal.(1992).Accordingtotheseauthors,theepitopehasbeenlocalizedonthealpha-helicalpartofvimentin(roddomaincoil2).Duetoanaasubstitutionatpositionofaa353inmurinevimentin(thatcouldexplainfortheweakcross-reactionoftheantibodywithmurinevimentin)theywereabletonarrowdownthebindingregionaroundposition353.Thesefindingswereconfirmedbytruncationmutagenesisexperimentsusinghumanvimentin(Rogersetal.,1995).Polypeptidereacting:MW57kDaintermediatefilamentprotein(vimentin)ofmesenchymalcells. |
| Isotype | IgG2a |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | AlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM] |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells. SIZE:466aminoacids;53652Da SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein. TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines. PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized. SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily. |
| MolecularWeight | 57kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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