Description | |
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CatalogueNumber | CBL434 |
BrandFamily | Chemicon® |
TradeName |
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Description | Anti-c-mycAntibody,clone3C7 |
ProductInformation | |
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Format | Purified |
Presentation | Themonoclonalispresentedataconcentrationof100μg/1mlinphosphatebufferedsalinecontaining10mMsodiumazideand1mg/mlbovineserumalbumin.Werecommendthateachlaboratorydetermineanoptimumworkingtitreforuseinitsparticularapplication. |
StorageandShippingInformation | |
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StorageConditions | Forusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C. |
Applications | |
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Application | Detectc-mycalsoknownasProto-oncogenec-MycwithAnti-c-mycAntibody,clone3C7(MouseMonoclonalAntibody),thathasbeendemonstratedtoworkinELISA,IP&WB. |
KeyApplications |
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ApplicationNotes | Westernblotting Immunoprecipitation Immunoassaydetectionofc-myc Optimalworkingdilutionsmustbedeterminedbytheenduser. |
BIOLOGicalInformation | |
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Immunogen | Syntheticpeptide:Cys-Ser-Thr-Ser-Ser-Leu-Tyr-Leu-Gln-Asp-Leu-Ser-Ala-Ala-Ala-Ser-Glu-Cysselectedfromthehumanc-mycsequence(aminoacids(171-188).Thepeptidewasconjugatedtokeyholelimpethaemocyanin. |
Epitope | a.a.171-188 |
Clone | 3C7 |
Host | Mouse |
Specificity | Inimmunoblottingthisantibodydetectsonemajorbandat62-64kDawhichcanbeblockedbypre-incubationwiththeimmunizingpeptide.Inimmunoprecipitationtheantibodydetectsa62kDabandfromcelllysates. KNOWNSPECIESCROSSREACTIVITY:Thisantibodyisknowntocrossreactwithmousec-myc |
Isotype | IgG1 |
SpeciesReactivity |
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AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Theproteinencodedbythisgeneisamultifunctional,nuclearphosphoproteinthatplaysaroleincellcycleprogression,apoptosisandcellulartransformation.Itfunctionsasatranscriptionfactorthatregulatestranscriptionofspecifictargetgenes.Mutations,overexpression,rearrangementandtranslocationofthisgenehavebeenassociatedwithavarietyofhematopoietictumors,leukemiasandlymphomas,includingBurkittlymphoma.Thereisevidencetoshowthatalternativetranslationinitiationsfromanupstream,in-framenon-AUG(CUG)andadownstreamAUGstartsiteresultintheproductionoftwoisoformswithdistinctN-termini.Thesynthesisofnon-AUGinitiatedproteinissuppressedinBurkitt"slymphomas,suggestingitsimportanceinthenormalfunctionofthisgene. |
GeneSymbol |
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UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P01106#Participatesintheregulationofgenetranscription.BindsDNAbothinanon-specificmannerandalsospecificallytorecognizesthecoresequence5"-CAC[GA]TG-3".Seemstoactivatethetranscriptionofgrowth-relatedgenes. SIZE:439aminoacids;48804Da SUBUNIT:EfficientDNAbindingrequiresdimerizationwithanotherbHLHprotein.BindsDNAasaheterodimerwithMAX.InteractswithTAF1CandSPAG9.InteractswithPARP10.InteractswithJARID1AandJARID1B. SUBCELLULARLOCATION:Nucleus. PTM:PhosphorylatedbyPRKDC. DISEASE:SwissProt:P01106#OverexpressionofMYCisimplicatedintheetiologyofavarietyofhematopoietictumors.&AchromosomalaberrationinvolvingMYCmaybeacauseofaformofB-cellchroniclymphocyticleukemia.Translocationt(8;12)(q24;q22)withBTG1. SIMILARITY:SwissProt:P01106##Contains1basichelix-loop-helix(bHLH)domain. |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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Millipore常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |