商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-1024 |
| Description | Anti-EGFRAntibody,cytoplasmicdomain |
| AlternateNames |
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| BackgroundInformation | EGFR(EpidermalGrowthFactorReceptor;ErbB-1;HER1)isthecell-surfacereceptorformembersoftheepidermalgrowthfactorfamily(EGF-family)ofextracellularproteinligands.TheEGFreceptorisamemberoftheErbBfamilyofreceptors,asubfamilyoffourcloselyrelatedreceptortyrosinekinases:EGFR(ErbB-1),HER2/c-neu(ErbB-2),Her3(ErbB-3)andHer4(ErbB-4).MutationsaffectingEGFRexpressionoractivitycouldresultincancer. EGFRdimerizationstimulatesitsintrinsicintracellularprotein-tyrosinekinaseactivity.Asaresult,autophosphorylationoffivetyrosine(Y)residues(Y992,Y1045,Y1068,Y1148andY1173)intheC-terminaldomainofEGFRoccurs.Thisautophosphorylationelicitsdownstreamactivationandsignalingbyseveralotherproteinsthatassociatewiththephosphorylatedtyrosinesthroughtheirownphosphotyrosine-bindingSH2domains. Thesedownstreamsignalingproteinsinitiateseveralsignaltransductioncascades,principallytheMAPK,AktandJNKpathways,leADIngtoDNAsynthesisandcellproliferation. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Presentation | Affinitypurifiedrabbitpolyclonalin0.1MTris-Glycine(pH7.4)with150mMNaCland0.05%NaN3. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution. |
| Applications | |
|---|---|
| Application | ThisAnti-EGFRAntibody,cytoplasmicdomainisvalidatedforuseinWBforthedetectionofEGFR. |
| KeyApplications |
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| BIOLOGicalInformation | |
|---|---|
| Immunogen | GSTfusionproteincorrespondingtothecytoplasmicdomainofhumanEGFR. |
| Epitope | CytoplasmicDomain |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | DetectsEGFR |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.Otherspecieshavenotbeentested. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Theproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.[providedbyRefSeq] |
| GeneSymbol |
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| PurificationMethod | ImmunogenAffinityPurified |
| UniProtNumber | |
| UniProtSummary | Function:ReceptorforEGF,butalsoforothermembersoftheEGFfamily,asTGF-alpha,amphiregulin,betacellulin,heparin-bindingEGF-likegrowthfactor,GP30andvacciniavirusgrowthfactor.Isinvolvedinthecontrolofcellgrowthanddifferentiation.PhosphorylatesMUC1inbreastcancercellsandincreasestheinteractionofMUC1withC-SRCandCTNNB1/beta-catenin.Ref.31 Isoform:2/truncatedisoformmayactasanantagoNIST.Ref.31 Catalyticactivity:ATP+a[protein]-L-tyrosine=ADP+a[protein]-L-tyrosinephosphate. Subunitstructure:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1.BindsMUC1.Ref.31Ref.25Ref.29Ref.30Ref.35 Subcellularlocation:Cellmembrane;Single-passtypeImembraneprotein. Isoform:2:Secreted. Tissuespecificity:Ubiquitouslyexpressed.Isoform2isalsoexpressedinovariancancers.Ref.38 Post-translationalmodification:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.Ref.16Ref.24Ref.28Ref.32Ref.33Ref.34Ref.36Ref.37Ref.39Ref.40Ref.41Ref.42 MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur. Involvementindisease:DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. Miscellaneous:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation. Sequencesimilarities:Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily. Contains1proteinkinasedomain. |
| MolecularWeight | 180kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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