Millipore/FCCH025142 | FlowCellect™ Cell Cycle Checkpoint H2A.X DNA Damage Kit/FCCH025142/25 Tests / Kit,Millipore,,Millipore,密理博,merckmillipore,Millipore经销商/代理商 millipore CA膜,聚丙烯膜,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Millipore/FCCH025142 | FlowCellect™ Cell Cycle Checkpoint H2A.X DNA Damage Kit/FCCH025142/25 Tests / Kit

产品编号：FCCH025142

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Millipore

公司：Millipore

公司分类：

商品介绍

Description
CatalogueNumber	FCCH025142
TradeName	FlowCellect
Description	FlowCellect™CellCycleCheckpointH2A.XDNADamageKit
Overview	Millipore’sFlowCellect™DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoidentifythelinkagebetweenDNAdamageinrelationtocellcyclecheckpointsinflowcytometryapplications.ThekitincludesadirectlyconjugatedAnti-phospho-HistoneH2A.X(Ser139)AlexaFluor®488antibodyplusaDNAdye(PropidiumIodide)whichallowstheresearchertoperformbivariateanalysistoidentifywhereDNAdamagetakesplacerelativetothepositioningwithinthecellcycle.Thekitincludesanoptimizedprotocolwithallofthenecessarycomponentstoprovidetheresearcheratrue“plugandplay”designfortheirresearchwhenstudyingDNAdamageandcellcyclecheckpoints.Thisimmunocytochemicalmethodisverysensitive,anditoffersanassaytoscreenanti-tumordrugsateveryphaseofthecellcycle. Inall,acomprehensiveunderstandingofDNAdamageanditsassociationwithcellcyclebehaviorcanprovidetheresearcherwithusefulinformationwhichwillbeimportantininterpretingtheintrinsicnatureofcellproliferation,apoptosis,DNArepair,andassistinthedevelopmentofanti-neoplasticagents.Millipore’sFlowCellect™DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoallowtheresearchertoeasilyidentify,discriminate,andquantitatecellsundergoingDNAdamagerelativetoitspositioningwithinthecellcycle. AllFlowCellectkitsareoptimizedonguava®benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudyDSBsandcellcycledistributionrightinthecomfortoftheirownlab.Thiskitcontainsoptimizedfixation,permeABIlization,wash,andflowbufferstoprovideresearcherswithacompletesolutionforbivariateanalysistoassessDNAdamageinrelationtopositioningwithinthecellcycle.
AlternateNames	FlowCellect
BackgroundInformation	DetectionofDNAdamageduetodoublestrandedbreaks(DSBs)isafundamentalmethodforassessingcellhealthandevaluationofanti-neoplasticagents.BivariateanalysismonitoringtheextentofH2A.XphosphorylationalongwithaDNAdyeoffersasensitiveandvaluabletoolinstudyingthefactorsthatinduceDNAdamage(measuredbythelevelofDSBs)and/oraffectDNArepair.UnderstandingwherethefirstindicationofDSBstakeplaceduringthecellcyclecanhelpresearchersunderstandthemechanismsinvolvedincellcyclearrestandDNArepairatcriticalcellcyclecheckpoints.MalfunctionsinthecellcycleinresponsetoDNAdamagecanincreasetheincidenceofcancersincetheDNArepairmachineryfailstooperateproperly.ThisisimportantbecausethecellhasseveralsystemsforinterruptingthecellcycleiftheDNArepairmachinerymalfunctions.EvidencesuggeststhatH2A.XphosphorylationmayrecruitcheckpointproteinstositesofDNAdamageandplayaroleinDNArepair. VariousfactorscancauseDNAdoublestrandedbreaksresultinginDNAdamage.PhysicalfactorssuchasUVandionizingrADIation,orchemicalfactorssuchassyntheticcompounds(anti-tumordrugs)canallcauseDNAdamage.RegardlessofthesourcetoinduceDNAdamage,theendresultwilleithercausecellcyclecheckpointarrest,apoptosis,orwilltriggertheDNArepairresponsetodamage.

ProductInformation
Components	1.20XAnti-p-HistoneH2A.X(Ser139),cloneJBW301-AlexaFluor®488Conjugate:(PartNo.CS206387)Onevialcontaining150µLantibody. 2.PropidiumIodideSolution:(PartNo.CS204836)Onevialcontaining400µLDNAdye. 3.RNaseAReagent:(PartNo.CS204821)Onevialcontaining25µL. 4.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mLbuffer. 5.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mLbuffer. 6.5XAssayBuffer:(PartNo.CS202124)Onebottlecontaining55mLbuffer. 7.1XPermeabilizationBuffer:(PartNo.CS203284)Twobottlescontaining14mLbuffer.
Detectionmethod	Fluorescent

ProductInformation

Components

1.20XAnti-p-HistoneH2A.X(Ser139),cloneJBW301-AlexaFluor®488Conjugate:(PartNo.CS206387)Onevialcontaining150µLantibody.
2.PropidiumIodideSolution:(PartNo.CS204836)Onevialcontaining400µLDNAdye.
3.RNaseAReagent:(PartNo.CS204821)Onevialcontaining25µL.
4.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mLbuffer.
5.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mLbuffer.
6.5XAssayBuffer:(PartNo.CS202124)Onebottlecontaining55mLbuffer.
7.1XPermeabilizationBuffer:(PartNo.CS203284)Twobottlescontaining14mLbuffer.

Detectionmethod

Fluorescent

StorageandShippingInformation
StorageConditions	4-8°Cforantibodiesandbuffers

Applications
Application	ThisFlowCellectDNADamage(HistoneH2A.X)&CellCycleAnalysisKitforFlowCytometryisdesignedtoidentifythelinkagebetweenDNAdamageinrelationtocellcyclecheckpointsinflowcytometryapplications.
KeyApplications	FlowCytometry

BIOLOGicalInformation
Clone	JBW301
Host	Mouse
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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