产品编号：FCCS025153
市 场 价：¥6940.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | FCCS025153 |
| TradeName |
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| Description | FlowCellect™DNADamageHistoneH2A.XDualDetectionKit |
| Overview | Millipore’sFlowCellect™DualDetectionkitsareaseriesofflowcytometryproductswhichincludeapairofantibodiesthatbindtothesameprotein;onetodetecttotalproteinexpressionandanothertodetectthephosphorylatedformofthesametarget.Usingtwoparameteranalysis,wecanachievetargetspecificdetectionofphosphorylationand,bydoingso,eliminatefalsepositiveswhileenhancingthesignaltonoiseratio. FlowCellect™HistoneH2A.XDNADamageDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-HistoneH2A.X(Ser139)-PerCPandanAnti-HistoneH2A.X-FITCconjugatedantibodytomeasuretotallevelsofHistoneH2A.X.ThistwocolorkitisdesignedtodetecttheextentofHistoneH2A.XpathwayactivationbymeasuringH2A.XphosphorylationrelativetothetotalH2A.Xexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofH2A.Xactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-HistoneH2A.Xconfirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedcellpopulation.UsingthisantibodypairprovidesasensitiveandvaluabletooltostudythefactorsthatinduceDNAdamageand/oraffectDNArepair,andallowonetoexplorethelinkagebetweenDNAdamage,cellcyclecheckpoints,andinitiationofapoptosis. |
| AlternateNames |
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| BackgroundInformation | H2A.XisamemberofthehistoneH2Afamily.HistoneH2A.XresidesdownstreamoftheDNAdamagekinasesignalingcascade.PhosphorylationofHistoneH2A.Xatserine139isanimportantindicatorofDNAdamage.AsthelevelofDNAdamageincreases,thelevelofphosphoHistoneH2A.X(alsoknownasγH2AX)increases,accumulatingatthesitesofDNAdamage.ThisaccumulationofphosphoHistoneH2A.XisoftenusedtoindicatethelevelofDNAdamagepresentwithinthecell.H2AXisalsoresponsIBLeforrecruitingresponseproteinstothesiteofDNAdamageandmayplayaroleinDNArepair. InordertovalidatethatthelevelofH2A.Xactivationisaccurate,atotalH2A.XantibodyismultiplexedbyflowcytometrytoallowthetotalandphospholevelstobenormalizedsinceDNAcontentlevelsvarybasedonwhichstagecellsarewithinthecellcycle.HistonedoublesincontentduringthecellcycleatthesamerateDNAcontentdoubles.HavingatotalH2A.Xwillaccountforthisincrease,sothephosphorylatedlevelscanbeaccuratelydetermined. Inall,acomprehensiveunderstandingofHistoneH2A.XactivityandunderstandingtheconsequenceofitsactivationcanprovidetheresearcherwithusefulinformationwhichwillbeimportantinunderstandingtheintrinsicnatureoftheDNAdamageresponse.Millipore’sFlowCellect™HistoneH2A.XDNADamageDualDetectionKitisdesignedtoallowtheresearchertomonitorandaccuratelymeasurephospho-specificHistoneH2A.Xactivationinapopulationofcells. |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Fluorescent |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 4-8°Cforantibodiesandbuffers |
| BIOLOGicalInformation | |
|---|---|
| Host | Mouse |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
Millipore常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |
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