Description | |
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CatalogueNumber | FCCS100182 |
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Description | FlowCellect™HistoneH2A.XPhosphorylationAssayKit |
Overview | Millipore’sFlowCellect™HistoneH2A.XPhosphorylationAssaykitincludesonedirectlyconjugatedantibody,aphospho-specificAnti-phospho-HistoneH2A.X(Ser139)-AlexaFluor®488conjugatedantibodytomeasurelevelsofHistoneH2A.X.ThiskitisdesignedtodetecttheextentofHistoneH2A.XpathwayactivationbymeasuringH2A.Xphosphorylationinanygivencellpopulation.ThekitprovidesasensitiveandvaluabletooltostudythefactorsthatinduceDNAdamageand/oraffectDNArepair,andallowonetoexplorethelinkagebetweenDNAdamage,cellcyclecheckpoints,andinitiationofapoptosis. IonizingrADIation(IR)andmanychemotherapeuticagentslikeetoposidekillcancercellsbyinductionofDNADSBs.Severalreportsshowthatthelevelofgamma-H2A.XasdetectedbyflowcytometrycorrelateswiththenumberofDNAstrandbreaks,tothelevelofcelldeathandradiosensitivity.H2A.XphosphorylatesinresponsetoaDNAdamagingreagent(e.g.Etoposide)orUVlight,anditsactivationclearlyindicatesthatDSBshaveoccurred.UnderstandingwhenDSBstakeplacecanhelpresearchersunderstandthemechanismsinvolvedinDNArepairandtheDNAdamageresponse. TheantibodyprovidedinthekithasbeencarefullytitratedtoensuretheABIlitytomeasurephospho-HistoneH2A.Xforaccuratedeterminationofproteinactivation.Sufficientreagentsareprovidedtoperform100tests.Detailedassayinstructionsareincludedtoassistinanalysisandtoensurethecorrectcellconcentrationisobtainedduringacquisitionofsampledata. |
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BackgroundInformation | H2A.XisamemberofthehistoneH2Afamily.HistoneH2A.XresidesdownstreamoftheDNAdamagekinasesignalingcascade.PhosphorylationofHistoneH2A.Xatserine139isanimportantindicatorofDNAdamage.AsthelevelofDNAdamageincreases,thelevelofphosphoHistoneH2A.X(alsoknownasγH2AX)increases,accumulatingatthesitesofDNAdamage.ThisaccumulationofphosphoHistoneH2A.XisoftenusedtoindicatethelevelofDNAdamagepresentwithinthecell.H2AXisalsoresponsIBLeforrecruitingresponseproteinstothesiteofDNAdamageandmayplayaroleinDNArepair. Inall,acomprehensiveunderstandingofHistoneH2A.XactivityandunderstandingtheconsequenceofitsactivationcanprovidetheresearcherwithusefulinformationwhichwillbeimportantinunderstandingtheintrinsicnatureoftheDNAdamageresponse.Millipore’sFlowCellectHistoneH2A.XPhosphorylationAssayKitisdesignedtoallowtheresearchertomonitorandaccuratelymeasurephospho-specificHistoneH2A.Xactivationinapopulationofcells. AllFlowCellectkitsareoptimizedonguava®benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudytheHistoneH2A.Xsignalingpathwayrightinthecomfortoftheirownlab.Thedirectlyconjugatedantibodyprovidedinthekithasbeencarefullytitratedandoptimizedtoensuremaximalperformancealleviatingtheneedforanyadditionaloptimization.Thiskitcontainsoptimizedfixation,permeabilization,wash,andassaybufferstoprovideresearcherswithacompletesolutionforHistoneH2A.Xsignalinganalysis. |
ProductInformation | |
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Components |
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Detectionmethod | Fluorescent |
StorageandShippingInformation | |
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StorageConditions | 4-8°Cforantibodiesandbuffers |
BIOLOGicalInformation | |
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Host | Mouse |
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AntibodyType | MonoclonalAntibody |
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MaterialsInformation |
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Millipore常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Millipore/475855-1R神奇滤布 密理博Millipore现货促销 | 475855-1R | Life Science Research | 膜 | 93.50 | Millipore/17-701/EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit | 17-701 | Life Science Research | 定量试剂盒 | 653.00 | Millipore/RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | 03-110 | Life Science Research | 抗体套装 | 433.00 | Millipore/CBL468F | Anti-PECAM-1 Antibody, clone HC1/6, FITC conjugated/CBL468F/100 assays | CBL468F | Antibodies and Assays | 功能性抗体 | 298.00 | Millipore/ECM460 | αVβ3/PECAM Investigator Kit/ECM460/1 kit | ECM460 | Antibodies and Assays | 夹心法ELISA | 477.00 |